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Primary Antibodies

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Catalog #1: bs-3330R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-3330R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-3330R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig

Conjugate: Cy3

Catalog #1: bs-3330R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-3330R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig

Conjugate: Cy7

Catalog #1: bs-3330R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig

Conjugate: FITC

Catalog #1: bs-3330R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-3330R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig

Conjugate: ALEXA FLUOR® 680

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-GLUT4 Polyclonal Antibody (bs-0384R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with Anti-GLUT4 Polyclonal Antibody (bs-0384R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
K562 cells probed with GLUT4 Polyclonal Antibody, unconjugated (bs-0384R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
Tissue\/cell: NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GLUT4) polyclonal Antibody, Unconjugated (bs-0384R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0384R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Sheep, Goat

Conjugate: Unconjugated

Catalog #1: bs-3292R-A350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Pig

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-3292R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Pig

Conjugate: Unconjugated

Catalog #1: bs-3292R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Pig

Conjugate: Biotin

Catalog #1: bs-3292R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Pig

Conjugate: HRP

Catalog #1: bs-3292R-A594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Pig

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-3292R-A488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Pig

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-3292R-A555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Pig

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-3292R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Pig

Conjugate: Cy3

Catalog #1: bs-3292R-A647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Pig

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-3292R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Pig

Conjugate: FITC

Catalog #1: bs-3292R-A680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Pig

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-3292R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Pig

Conjugate: Cy7

Catalog #1: bs-3292R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Pig

Conjugate: Cy5

Catalog #1: bs-3292R-A750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Pig

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-3425R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: ALEXA FLUOR® 350

Lane 1: mouse embryo lysates Lane 2: mouse brain lysates probed with Anti Phospho-Smad3(Ser423\/425) Polyclonal Antibody, Unconjugated (bs-3425R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 47kD. Observed band size: 47kD.
Formalin-fixed and paraffin embedded human liver carcinoma labeled with Rabbit Anti-Smad3 (Ser423 + Ser425) Polyclonal Antibody (bs-3425R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad3 (Ser423 + Ser425) Polyclonal Antibody(bs-3425R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad3 (Ser423 + Ser425) Polyclonal Antibody, Unconjugated (bs-3425R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3425R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-3425R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Biotin

Catalog #1: bs-3425R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig

Conjugate: HRP

Catalog #1: bs-3425R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-3425R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-3425R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy5

Catalog #1: bs-3425R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-3425R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy3

Catalog #1: bs-3425R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-3425R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy7

Catalog #1: bs-3425R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: FITC

Catalog #1: bs-3425R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-3425R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: ALEXA FLUOR® 750

Formalin-fixed and paraffin embedded mouse lung labeled with (bs-0812R) Rabbit Anti-IL-1 Beta\/IL-1B Polyclonal Antibody, Unconjugated followed by conjugation to the secondary antibody and DAB staining.
Image was kindly submitted by Dr.Rachel Reith, NIH. Paraffin-embedded mouse brain stained with Rabbit Anti-IL-1 Beta Polyclonal Antibody,1:100 overnight at 4C.\\n
Raw264.7 lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin-embedded Human Cervical Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4\u00b0C. Followed by incubation with the secondary antibody, DAB and counterstaining.

Catalog #1: bs-0812R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), dot-blot

Reactivity: Human, Mouse, Rat, Bovine, Pig, Rabbit, Others

Conjugate: Unconjugated

Catalog #1: bs-0061R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Cy5

Catalog #1: bs-0061R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: HRP

Catalog #1: bs-0061R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Biotin

Catalog #1: bs-0061R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-0061R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-0061R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-0061R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-0061R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-0061R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Cy3

Catalog #1: bs-0061R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: ALEXA FLUOR® 680