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Primary Antibodies

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Catalog #1: bs-4605R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rabbit

Conjugate: HRP

Catalog #1: bs-4605R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rabbit

Conjugate: Cy5

Catalog #1: bs-4605R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rabbit

Conjugate: FITC

Catalog #1: bs-5437R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bs-5437R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bs-5437R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: Biotin

Catalog #1: bs-5437R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cy3

Catalog #1: bs-5437R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cy5

Catalog #1: bs-5437R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cy7

Catalog #1: bs-5437R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-6319R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Raw264.7 Cells (30ug); Lane 2: Mouse Liver (40ug); Probed with IL-1 Beta Polyclonal Antibody, unconjugated (bs-6319R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Raw264.7 cell lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-6319R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL-1 Beta Polyclonal Antibody(bs-6319R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-6319R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-6319R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Biotin

Catalog #1: bs-6319R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: HRP

Catalog #1: bs-6319R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy3

Catalog #1: bs-6319R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy5

Catalog #1: bs-6319R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy7

Catalog #1: bs-6319R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: FITC

Catalog #1: bs-6417R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: HRP

Lane 1: human colon carcinoma lysates Lane 2: mouse brain lysates probed with Anti phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 110-123kD. Observed band size: 115,120kD.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-phospho-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-PI3 Kinase p110 beta(Ser1070) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human MCF-7 cell lysates; Lane 2: Human K562 cell lysates probed with phospho-PI3 Kinase p110 beta (Ser1070) Polyclonal Antibody, Unconjugated (bs-6417R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6417R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-6417R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Biotin

Catalog #1: bs-6417R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Cy5

Catalog #1: bs-6417R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Cy3

Catalog #1: bs-6417R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: FITC

Catalog #1: bs-6417R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Cy7

Catalog #1: bs-0920R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: HRP

Catalog #1: bs-1251R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rabbit

Conjugate: FITC

Catalog #1: bs-0734R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Rabbit

Conjugate: Cy7

Catalog #1: bs-10200R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy3

Catalog #1: bs-10200R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy5

Catalog #1: bs-1501R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy5

Catalog #1: bs-2044R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bs-6640R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bs-6640R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-6640R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: HRP

Catalog #1: bs-6640R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: FITC

Catalog #1: bs-6640R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy3

Catalog #1: bs-6640R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy5

Catalog #1: bs-6640R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy7

Catalog #1: bs-6645R-Biotin

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Mouse brain lysates probed with Rabbit Anti-WNT7A Polyclonal Antibody, Unconjugated (bs-6645R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WNT7A Polyclonal Antibody, Unconjugated (bs-6645R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Mouse Kidney tissue lysates ; Lane 5: Rat Placenta tissue lysates; Lane 6: Rat Lung tissue lysates; Lane 7: Rat Testis tissue lysates ; Lane 8: Rat Kidney tissue lysates ; Lane 9: Human Hela cell lysates ; Lane 10: Human A431 cell lysates ; Lane 11: Human SH-SY5Y cell lysates ; Lane 12: Human HepG2 cell lysates probed with WNT7A Polyclonal Antibody, Unconjugated (bs-6645R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6645R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6645R-HRP

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-6645R-FITC

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-6645R-Cy3

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-6645R-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-6645R-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-7552R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bs-7552R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: HRP

Paraformaldehyde-fixed (4%) Human Endothelial Cells were blocked with normal goat serum for 20mins at 37\u2103. The cells were then labeled with Biglycan Polyclonal Antibody, Unconjugated (bs-7552R) at 1:50, overnight at 4\u00b0C. Secondary antibody incubation was performed with Goat Anti-Rabbit IgG, Rhodamine B conjugated antibody (bs-0295G-RBITC) at a 1:200 dilution for 40 minutes at 37\u00b0C. DAPI (5ug\/ml; blue) was used to stain the cell nuclei
Formalin-fixed and paraffin-embedded Human Lung Carcinoma labeled with Biglycan Polyclonal Antibody, Unconjugated (bs-7552R) at 1:200 followed by secondary antibody, DAB and counterstaining
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Biglycan Polyclonal Antibody (bs-7552R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Mouse Bone probed with Biglycan Polyclonal Antibody (bs-7552R) at 1:300, overnight at 4˚C. Followed by incubation with a conjugated secondary antibody.

Catalog #1: bs-7552R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated