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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bs-0195R-Cy7

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-0195R-Cy5

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-0195R-Cy5.5

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-0195R-FITC

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-0195R-PE

Applications: WB, FCM, IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bs-8721R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-8721R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-8721R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-8721R-Cy3

Applications: IF(IHC-P), IF(ICC)

Reactivity: Rat

Conjugate: Cy3

Catalog #1: bs-8721R-Cy5.5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Rat

Conjugate: Cy5.5

Catalog #1: bs-8721R-Cy5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Rat

Conjugate: Cy5

Catalog #1: bs-8721R-FITC

Applications: IF(IHC-P), IF(ICC)

Reactivity: Rat

Conjugate: FITC

Catalog #1: bs-8721R-Cy7

Applications: IF(IHC-P), IF(ICC)

Reactivity: Rat

Conjugate: Cy7

Catalog #1: bs-8747R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-8747R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-8747R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-8747R-Cy3

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-8747R-Cy5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-8747R-Cy7

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-8747R-Cy5.5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-8747R-FITC

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: FITC

Formalin-fixed and paraffin embedded rat kidney tissue labeled withAnti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032r) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse embryo lysates, L2 mouse brain lysates probed with Anti- Bcl-2 Polyclonal Antibody, Unconjugated (bs-0032R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. P

Catalog #1: bs-0032r

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-0078P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded human colitis tissue labeled with Anti-TNF-alpha Polyclonal Antibody, Unconjugated(bs-0077R) 1:300 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded human colitis tissue labeled with Anti-TNF-alpha Polyclonal Antibody, Unconjugated(bs-0077R) 1:300 followed by conjugation to the secondary antibody and DAB staining\\n
Human liver lysates probed with Anti TNF alpha Polyclonal Antibody, Unconjugated (bs-0078R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 17kD, 26kD. Observed band size:26kD.

Catalog #1: bs-0078R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0078R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0078R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-0078R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-0078R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-0078R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bs-0078R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-0078R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-0636R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Cyclin D1 Polyclonal Antibody, Unconjugated (bs-0623R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
A549 cells probed with Anti-Cyclin D1 Polyclonal Antibody (bs-0623R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
Image provided by One World Lab validation program. HEK293 cells probed with Rabbit Anti-Cyclin D1 Polyclonal Antibody (bs-0623R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Cyclin D1 Polyclonal Antibody (bs-0623R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-0623r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0623R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0623R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0623R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-0623R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-0623R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-0623R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-0623R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-0623R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse placenta lysates probed with Anti-Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad2 Polyclonal Antibody, Unconjugated (bs-0718R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0718R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human rectal cancer labeled with Anti-Snail\/SNAI 1 Polyclonal Antibody, Unconjugated (bs-1371R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human pancreas carcinoma labeled with Rabbit Anti-Snail Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin-embedded human laryngocarcinoma labeled with Rabbit Anti-Snail Polyclonal Antibody, Unconjugated(bs-1371R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.\\n
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Snail1\/2\/3 Polyclonal Antibody(bs-1371R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1371r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP-1 Polyclonal Antibody, Unconjugated (bs-4597R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP-1 Polyclonal Antibody, Unconjugated (bs-4597R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Rat Testis lysates; Lane 2: Mouse Testis lysates probed with MMP-1 Polyclonal Antibody, Unconjugated (bs-4597R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP-1 Polyclonal Antibody, Unconjugated (bs-4597R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-4597R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4597R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-4597R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-4597R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-4597R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-4597R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC