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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Lane 1: HepG2 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH\/3T3 cell lysates probed with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-51733M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: PC-12 cell lysates; Lane 4: NIH\/3T3 cell lysates; Lane 5: HepG2 cell lysates probed with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-51733M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51733m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51737m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51740m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51738m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Human THP-1(LPS) cell lysates; Lane 2: Human LOVO cell lysates probed with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (bsm-51741M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with PTGS2\/Cyclooxygenase 2 monoclonal antibody, unconjugated (bsm-51741M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-51741m-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51742m

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51743m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7 cell lysates; Lane 2: MDA-MB-468 cell lysates probed with SULT2B1 Monoclonal Antibody, Unconjugated (bsm-51745M) at 1:8000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mcf-7 cell lysates probed with SULT2B1 Monoclonal Antibody, Unconjugated (bsm-51745M) at 1:8000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51745m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51744m

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51746m-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HepG2 cell lysates; Lane 2: Human Lung lysates; Lane 3: Human Liver lysates; Lane 4: Mouse Liver lysates; Lane 5: Mouse Kidney lysates; Lane 6: Rat Kidney lysates probed with SULT1A1 Monoclonal Antibody, Unconjugated (bsm-51747M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates probed with SULT1A1 Monoclonal Antibody, Unconjugated (bsm-51747M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51747m

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-51748M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-51748M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-51748M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-51748M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-51748m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HL-60 cell lysates; Lane 2: THP-1 cell lysates; Lane 3: K562 cell lysates probed with CARD9 Monoclonal Antibody, Unconjugated (bsm-51749M) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K62 cell lysates; Lane 2: HL-60 cell lysates probed with CARD9 Monoclonal Antibody, Unconjugated (bsm-51749M) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51749m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Hela cell lysates; Lane 2: A549 cell lysates; Lane 3: CCRF-CEM cell lysates; Lane 4: CHO cell lysates probed with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-51750M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: DU145 cell lysates; Lane 2: U-251MG cell lysates probed with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-51750M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51750m

Applications: WB

Reactivity: Human, Hamster

Conjugate: Unconjugated

Catalog #1: bsm-51752m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51751m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51753m

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51758m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51757m

Applications: WB

Reactivity: Recombinant protein

Conjugate: Unconjugated

Catalog #1: bsm-51759m

Applications: WB

Reactivity: Human, Mouse, Hamster

Conjugate: Unconjugated

Catalog #1: bsm-51756m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HepG2 cell lysates probed with THAP11 Monoclonal Antibody, Unconjugated (bsm-51505M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: NIH\/3T3 cell lysates probed with THAP11 Monoclonal Antibody, Unconjugated (bsm-51505M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51505m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with ERK2 monoclonal antibody, unconjugated (bsm-51555M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-ERK2 antibody (bsm-51555M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-51555m-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human astroglioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UCHL1\/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UCHL1\/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: 293T cell lysates; Lane 2: NCI-H1299 cell lysates; Lane 3: PC-12 cell lysates probed with UCHL1\/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51558m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Xenopus laevis

Conjugate: Unconjugated

Catalog #1: bsm-51556m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51673m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Monkey brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Zebrafish brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YY1(Nuclear Loading Control) Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: Daudi cell lysates; Lane 2: U251 cell lysates probed with YY1 Monoclonal Antibody, Unconjugated (bsm-51566M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51566m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-51701m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51703m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41411M-HRP

Applications: WB, ELISA, IHC-P

Reactivity: SARS-CoV-2

Conjugate: HRP

Catalog #1: bsm-41411M-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: SARS-CoV-2

Conjugate: Biotin

Catalog #1: bsm-33128M

Applications: WB

Reactivity: Plant

Conjugate: Unconjugated

Catalog #1: bsm-33128M-Biotin

Applications: WB

Reactivity: Plant

Conjugate: Biotin

Catalog #1: bsm-33128M-HRP

Applications: WB

Reactivity: Plant

Conjugate: HRP

Catalog #1: bsm-33260M

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33260M-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33260M-HRP

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (bsm-54472R) at 1200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (bsm-54472R) at 1200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PD-L1) Monoclonal Antibody, Unconjugated (bsm-54472R) at 1200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with PD-L1 monoclonal antibody, unconjugated (bsm-54472R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-54472r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33112M

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33112M-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33112M-HRP

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-2235M-HRP

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANO1\/TMEM16A Monoclonal Antibody, Unconjugated (bsm-49061M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-49061M

Applications: IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-49061M-Biotin

Applications: IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-49061M-HRP

Applications: IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-2235M

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-8770M

Applications: WB

Reactivity: Caenorhabditis Elegans

Conjugate: Unconjugated

Catalog #1: bsm-2235M-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin