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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bsm-2182M-FITC

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: FITC

Catalog #1: bsm-2271M-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Virus, Others

Conjugate: Biotin

Catalog #1: bsm-2271M-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Virus, Others

Conjugate: HRP

Catalog #1: bsm-2279M

Applications: ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-2271M

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bsm-2279M-Cy5

Applications: IF

Reactivity: Human, Rat, Rabbit

Conjugate: Cy5

Catalog #1: bsm-2279M-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bsm-2279M-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Rabbit

Conjugate: HRP

Catalog #1: bsm-2279M-Cy3

Applications: IF

Reactivity: Human, Rat, Rabbit

Conjugate: Cy3

Catalog #1: bsm-2279M-Cy7

Applications: IF

Reactivity: Human, Rat, Rabbit

Conjugate: Cy7

Catalog #1: bsm-2279M-FITC

Applications: IF

Reactivity: Human, Rat, Rabbit

Conjugate: FITC

Catalog #1: bsm-33085m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C-erbB-2\/HER2 Monoclonal Antibody, Unconjugated (bsm-33085M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C-erbB-2\/HER2 Monoclonal Antibody, Unconjugated (bsm-33085M) at 1:400 overnight at 4\u00b0C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with C-erbB-2\/HER2 monoclonal antibody, unconjugated (bsm-33085M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33085m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33085m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TUBB3 Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TUBB3 Monoclonal Antibody, Unconjugated (bsm-33177M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TUBB3 (Neuronal Marker) ) Monoclonal Antibody, Unconjugated (ascites of bsm-33177M 6F12) at 1:2000 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TUBB3 (Neuronal Marker) ) Monoclonal Antibody, Unconjugated (ascites of bsm-33177M 6F12) at 1:2000 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

Catalog #1: bsm-33177m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33177m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33177m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33236M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33236M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33236M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:300 overnight at 4\u00b0C, followed by Goat Anti-Mouse IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Lane 1: Cerebrum lysates probed with Bax Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bs-33279M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Bax ) Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33279M

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-33279M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-33279M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-33279M-Cy5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-33279M-Cy3

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-33279M-Cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-33279M-FITC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Paraformaldehyde-fixed, paraffin-embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Gamma Tubulin Monoclonal Antibody, Unconjugated (bsm-33313M) at 1:2000 overnight at 4\u00b0C, followed by DAB staining.
Paraformaldehyde-fixed, paraffin-embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Gamma Tubulin) Monoclonal Antibody, Unconjugated (bsm-33313M) at 1:2000 overnight at 4\u00b0C, followed by DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Gamma Tubulin Monoclonal Antibody(bsm-33313M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
25 ug total protein per lane of various lysates (see on figure) probed with Gamma Tubulin monoclonal antibody, unconjugated (bsm-33313M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33313m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33313m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33313m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33314M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-33314M-Biotin

Applications: WB

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-33314M-HRP

Applications: WB

Reactivity: Human, Mouse

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
RSC96 probed with alpha Tubulin (4G1) Monoclonal Antibody, Unconjugated (bsm-50179M) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human cervical carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Hela; Lane 2: 293T; Lane 3: MDA-MB-231; Lane 4: A549; Lane 5: A431; Lane 6: RSC96; Lane 7: VSC4.1; Lane 8: ASPC-1; Lane 9: B16; Lane 10: BV2; Lane 11: H9C2; Lane 12: Hep2G;Lane 13: K562; Lane 14: U937; probed with alpha Tubulin (4G1) Monoclonal Antibody, unconjugated (bsm-50179M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.\r\n

Catalog #1: bsm-50179M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat, All

Conjugate: Unconjugated

Lane 1 : HeLa Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : C6 Cell Lysate; probed with bsm-50240M p90RSK1 (8C12) Monoclonal Antibody at 2.0ug\/mL (1:500) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.
Immunoprecipitation of p90RSK1 from A431 cell line using p90RSK1 (8C12) Monoclonal Antibody (bsm-50240M). Lane 1 : A431 Cell Lysate; Lane 2 : Precipitated from 200ug of A431 Cell Lysate using 2ug of antibody; Lane 3 : Precipitated from 200ug of A431 Cell Lysate using 5ug of antibody; Lane 4 : Precipitated from PBS using 5ug of antibody. WB analysis was performed using bsm-50240M at 1.0ug\/mL (1:1000) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-50240M

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-50258m

Applications: WB, ELISA, IHC, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-33050m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33050m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Lane 1: Hela KO EGFR cell lysates; Lane 2: Hela cell lysates probed with EGFR Monoclonal Antibody, Unconjugated (bsm-33050M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with EGFR monoclonal antibody, unconjugated (bsm-33050M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with EGFR monoclonal antibody, unconjugated (bsm-33050M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33050m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1 : MCF7 Cell Lysate; Lane 2 : A431 Cell Lysate; Lane 3 : C6 Cell Lysate; Lane 4 : L929 Cell Lysate; probed with using bsm-50375M at Cyclin D1 (2G5) Monoclonal Antibody 4.0ug\/mL (1:250) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.
Lane 1 : Cyclin B1 Recombinant Protein; Lane 2 : Cyclin D1 Recombinant Protein; Lane 3 : Cyclin D2 Recombinant Protein; Lane 4 : Cyclin D3 Recombinant Protein; Lane 5 : Cyclin E1 Recombinant Protein; Lane 6 : Cyclin E2 Recombinant Protein; probed with using bsm-50375M at Cyclin D1 (2G5) Monoclonal Antibody 0.5ug\/mL (1:2000) and incubated at 4\u2103 overnight, followed by secondary antibody incubation for 60min at room temperature.

Catalog #1: bsm-50375M

Applications: WB, IP

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51181M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: L6; Lane 2: NIH\/3T3; Lane 3: T47D; Lane 4: A2058; Lane 5: HepG2; Lane 6: K562; Lane 7: HeLa Cell lysates; probed with Hsp90 (3C9) Monoclonal Antibody, unconjugated (bsm-51215M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Placenta tissue lysates; Lane 4: Rat Cerebrum tissue lysates ; Lane 5: Rat Placenta tissue lysates ; Lane 6: Human U251 cell lysates ; Lane 7: Human Molt-4 cell lysates ; Lane 8: Human HL60 cell lysates ; Lane 9: Human A549 cell lysates probed with HSP90 Monoclonal Antibody, Unconjugated (bsm-51215M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51215m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51261M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

HeLa Cell lysates; probed with PPT1 (10G4) Monoclonal Antibody, unconjugated (bsm-51262M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HepG2 Cell lysates; probed with PPT1 (10G4) Monoclonal Antibody, unconjugated (bsm-51262M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human cerebellum tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PPT1 (10G4) Monoclonal Antibody (bsm-51262M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
HepG2 cells probed with using PPT1 (10G4) Monoclonal Antibody (green) unconjugated bsm-51262M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG (blue).

Catalog #1: bsm-51262M

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human tonsil tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with FYN (2C7) Monoclonal Antibody (bsm-51263M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
293 cells probed with FYN (2C7) Monoclonal Antibody, unconjugated (bsm-51263M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Human Hela Cells probed with FYN (2C7) Monoclonal Antibody, Unconjugated (bsm-51263M) (green) at 1:25 for 60 minutes followed by a Alexa Fluor\u00ae 488 goat anti-mouse lgG at 1:400. The graph shows FYN staining compared a mouse IgG1 isotype control (blue).

Catalog #1: bsm-51263M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7 Cell lysates; Lane 2: U266 Cell lysates; probed with CDKN1B (10B7) Monoclonal Antibody, unconjugated (bsm-51281M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDKN1B (10B7) Monoclonal Antibody (bsm-51281M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51281M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MAFK Cell lysates; probed with MAFK (8C5) Monoclonal Antibody, unconjugated (bsm-51274M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells probed with using MAFK (8C5) Monoclonal Antibody (green) unconjugated bsm-51274M at 1:25 for 30 minutes followed by A488 secondary antibody at 1:400 compared to an isotype control of mouse IgG1(blue).

Catalog #1: bsm-51274M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51276M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated