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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bsm-30095m

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30096m-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (human CD34) Monoclonal Antibody, Unconjugated (bsm-30097M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD34 ) Monoclonal Antibody, Unconjugated (bsm-30097M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
TF-1 cells were\u00a0incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with human CD34 Antibody(bsm-30097M)at 1:200 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bsm-30097m-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30099m-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2 : Bosc23 cell lysate transfected with Myc\/His\/HePTP; Lane 3 : Bosc23 cell lysate transfected with GFP\/Ub+1 lysates, probed with HA tag (7H5) Monoclonal Antibody, unconjugated (bsm-50131M) at 1:10000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of Bosc23 cell lysates using HA tag (7H5) \r\nMonoclonal Antibody (bsm-50131M): Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2: Precipitated from 200ug Bosc23 transfected with HA\/HSP60 using 5ug of antibody; Lane 3 : Precipitated from PBS using 5ug of antibody; WB analysis was performed using bsm-50131M at 0.1ug\/mL (1:10000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #1: bsm-50131M

Applications: WB, IF(ICC), IP

Reactivity: Human, All

Conjugate: Unconjugated

Catalog #1: bsm-33865M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41179M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41179M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41179M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33855m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60284M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60497m-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60520m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-G3BP1 antibody (bsm-60465M,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with G3BP1 monoclonal antibody, unconjugated (bsm-60465M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-60465m-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60087M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa\uff0cNeuro-2a, BRL, Mouse spleen, Mouse kidney\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 15 kDa\r\nObserved MW: 15 kDa
Tissue: Human liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60091M
Tissue: Human colon cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60091M
Tissue: Mouse kidney\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60091M

Catalog #1: bsm-60091m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-41519R

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41476M

Applications: WB, ELISA

Reactivity: Mouse, Virus, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41627M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41631M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41635M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41634M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41632M

Applications: ELISA

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bsm-41615M

Applications: WB, ELISA

Reactivity: Mouse, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41630M

Applications: WB, ELISA

Reactivity: Mouse, Virus, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41516M

Applications: WB

Reactivity: Mouse, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41637M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41540M

Applications: ELISA

Reactivity: Monkey

Conjugate: Unconjugated

Catalog #1: bsm-41539M

Applications: ELISA

Reactivity: Monkey

Conjugate: Unconjugated

Catalog #1: bsm-41541m

Applications: ELISA

Reactivity: Monkey

Conjugate: Unconjugated

Catalog #1: bsm-0945m-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bsm-0945m-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-405

Catalog #1: bsm-0945m-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bsm-0945m-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bsm-0945m-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bsm-0945m-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bsm-0945m-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bsm-0945m-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-680

Catalog #1: bsm-0945m-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-750

Catalog #1: bsm-0945m-apc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bsm-0945m-apc-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bsm-0945m-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bsm-0945m-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5

Catalog #1: bsm-0945m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5.5

Catalog #1: bsm-0945m-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy7

Catalog #1: bsm-0945m-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PerCP

Catalog #1: bsm-0945m-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-0945m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: RBITC