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Primary Antibodies



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Catalog #1: bsm-30099m

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2 : Bosc23 cell lysate transfected with Myc\/His\/HePTP; Lane 3 : Bosc23 cell lysate transfected with GFP\/Ub+1 lysates, probed with HA tag (7H5) Monoclonal Antibody, unconjugated (bsm-50131M) at 1:10000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of Bosc23 cell lysates using HA tag (7H5) \r\nMonoclonal Antibody (bsm-50131M): Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2: Precipitated from 200ug Bosc23 transfected with HA\/HSP60 using 5ug of antibody; Lane 3 : Precipitated from PBS using 5ug of antibody; WB analysis was performed using bsm-50131M at 0.1ug\/mL (1:10000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at Room Temperature.

Catalog #1: bsm-50131M

Applications: WB, IF(ICC), IP

Reactivity: Human, All

Conjugate: Unconjugated

Catalog #1: bsm-33865M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41179M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41179M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-41179M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33855m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60284M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60497m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60520m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-G3BP1 antibody (bsm-60465M,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with G3BP1 monoclonal antibody, unconjugated (bsm-60465M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-60465m

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60087M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa\uff0cNeuro-2a, BRL, Mouse spleen, Mouse kidney\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 15 kDa\r\nObserved MW: 15 kDa
Tissue: Human liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60091M
Tissue: Human colon cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60091M
Tissue: Mouse kidney\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60091M

Catalog #1: bsm-60091m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-41519R

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41476M

Applications: WB, ELISA

Reactivity: Mouse, Virus, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41627M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41631M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41635M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41634M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41632M

Applications: ELISA

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bsm-41615M

Applications: WB, ELISA

Reactivity: Mouse, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41630M

Applications: WB, ELISA

Reactivity: Mouse, Virus, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41516M

Applications: WB

Reactivity: Mouse, SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41637M

Applications: ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41540M

Applications: ELISA

Reactivity: Monkey

Conjugate: Unconjugated

Catalog #1: bsm-41539M

Applications: ELISA

Reactivity: Monkey

Conjugate: Unconjugated

Catalog #1: bsm-41541m

Applications: ELISA

Reactivity: Monkey

Conjugate: Unconjugated

Catalog #1: bsm-0945m-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bsm-0945m-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-405

Catalog #1: bsm-0945m-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bsm-0945m-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bsm-0945m-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bsm-0945m-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bsm-0945m-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bsm-0945m-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-680

Catalog #1: bsm-0945m-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-750

Catalog #1: bsm-0945m-apc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bsm-0945m-apc-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bsm-0945m-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bsm-0945m-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5

Catalog #1: bsm-0945m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5.5

Catalog #1: bsm-0945m-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy7

Catalog #1: bsm-0945m-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PerCP

Catalog #1: bsm-0945m-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-0945m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: RBITC

Catalog #1: bsm-1240M-BF350

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-1240M-BF405

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-1240M-BF555

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-555