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Catalog #1: bsm-51474m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human prostate cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT2 Monoclonal Antibody, Unconjugated (bsm-51473M) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse brain tissue lysates probed with AKT2 Monoclonal Antibody, Unconjugated (bsm-51473M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Jurkat cell lysates; Lane 2: Hela cell lysates; Lane 3: Mouse brain tissue lysates; Lane 4: C6 cell lysates probed with AKT2 Monoclonal Antibody, Unconjugated (bsm-51473M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51473m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51478m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51493m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51499m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: HL-60 cell lysates probed with RCC1 Monoclonal Antibody, Unconjugated (bsm-51500M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HL-60 cell lysates; Lane 2: Hela cell lysates; Lane 3: U-251MG cell lysates; Lane 4: MCF-7 cell lysates; Lane 5: Mouse Heart tissue lysates probed with RCC1 Monoclonal Antibody, Unconjugated (bsm-51500M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51500m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51502m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51506m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: K562 cell lysates probed with RPS6KA3 Monoclonal Antibody, Unconjugated (bsm-51509M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Heart tissue lysates probed with RPS6KA3 Monoclonal Antibody, Unconjugated (bsm-51509M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51509m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51510m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51513m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51516m

Applications: WB, ELISA, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51514m

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: K562 cell lysates probed with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human liver tissue lysates; Lane 2: Hela cell lysates; Lane 3: HepG2 cell lysates; Lane 4: MCF-7 cell lysates;Lane 5: Neuro-2a cell lysates; Lane 6: PC-12 cell lysates probed with PSMA5 Monoclonal Antibody, Unconjugated (bsm-51520M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51520m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51517m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51521m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51526m

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-51528m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51533m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51524m

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-51535m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51537m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51538m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51541m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51545m

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51549m

Applications: WB

Reactivity: Recombinant protein

Conjugate: Unconjugated

Catalog #1: bsm-51550m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51559m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51561m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51562m

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51567m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51569m

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51568m

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BCL10 Monoclonal Antibody(bsm-51578M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BCL10 Monoclonal Antibody, Unconjugated(bsm-51578M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BCL10 Monoclonal Antibody, Unconjugated(bsm-51578M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BCL10 Monoclonal Antibody, Unconjugated(bsm-51578M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51578m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51588m

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51593m

Applications: WB, Flow-Cyt

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51607m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51636m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51649m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51658m

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD10) Monoclonal Antibody, Unconjugated (bsm-51667M) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD10) Monoclonal Antibody, Unconjugated (bsm-51667M) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with CD10 monoclonal antibody, unconjugated (bsm-51667M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD10 Monoclonal Antibody, Unconjugated (bsm-51667M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-51667m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51688m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51723m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-51748M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-51748M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-51748M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-51748M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-51748m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human astroglioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UCHL1\/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UCHL1\/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: 293T cell lysates; Lane 2: NCI-H1299 cell lysates; Lane 3: PC-12 cell lysates probed with UCHL1\/PGP9.5 Monoclonal Antibody, Unconjugated (bsm-51558M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51558m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Xenopus laevis

Conjugate: Unconjugated

Catalog #1: bsm-51556m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51701m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51703m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated