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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bsm-51233M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: MCF-7 Cell lysates; probed with MGMT (888CT22.2.4) Monoclonal Antibody, unconjugated (bsm-51234M) at 1:25 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human small intestine tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MGMT (888CT22.2.4) Monoclonal Antibody (bsm-51234M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51234M

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51235M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51237M

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD73) Monoclonal Antibody, Unconjugated (bsm-51239M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD73) Monoclonal Antibody, Unconjugated (bsm-51239M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD73) Monoclonal Antibody, Unconjugated (bsm-51239M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD73) Monoclonal Antibody, Unconjugated (bsm-51239M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-51239m-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51242M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse Adipose tissue lysates; Lane 2: Mouse Breast tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Thymus tissue lysates probed with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Fat; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51247m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51243M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51244M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51250M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51251M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51252M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51259M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51255M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51254M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51256M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with NFKB1 (8C3) Monoclonal Antibody (bsm-51258M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with NFKB1 (8C3) Monoclonal Antibody (bsm-51258M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51258M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51269M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

KLF4 recombinant protein (20ug) probed with bsm-51264M KLF4 (5A1) Monoclonal Antibody at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: KLF4 protein; probed with KLF4 (5A1) Monoclonal Antibody, unconjugated (bsm-51264M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51264M

Applications: WB, IF(ICC)

Reactivity:

Conjugate: Unconjugated

Lane 1: U-251 MG Cell lysates; probed with EPHA5 (46CT61.6.4) Monoclonal Antibody, unconjugated (bsm-51268M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with EPHA5 (46CT61.6.4) Monoclonal Antibody (bsm-51268M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51268M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293T; Lane 2: A431 probed with bsm-51265M PINK1 (38CT20.8.5) Monoclonal Antibody at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PINK1 (38CT20.8.5) Monoclonal Antibody (bsm-51265M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PINK1 (38CT20.8.5) Monoclonal Antibody (bsm-51265M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51265m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with SOD2 (37CT127.5.11.6) Monoclonal Antibody (bsm-51267M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SOD2 (6C11) Monoclonal Antibody, Unconjugated (bsm-51267M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SOD2 (6C11) Monoclonal Antibody, Unconjugated (bsm-51267M) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Siha cell lysates; Lane 2: HepG2 cell lysates; Lane 3: K562 cell lysates; Lane 4: U251 cell lysates probed with SOD2 (6C11) Monoclonal Antibody, Unconjugated (bsm-51267M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51267M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51324m

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51271M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51323M

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51393M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Sample Tissue\/Cell lysates; Lane 2: Sample Tissue\/Cell lysates; Lane 3: Sample Tissue\/Cell lysates; probed with antibodyname Monoclonal Antibody, unconjugated (bsm-51388M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Jurkat Cell lysates; Lane 2: MCF-7 Cell lysates; Lane 3: NIH\/3T3 Cell lysates; probed with RPS6KB2 (164CT21.2.2) Monoclonal Antibody, unconjugated (bsm-51388M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51388M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Human Liver Lysates; Lane 2: MCF-7 Cell Lysates; Lane 3: HepG2 Cell Lysates; Lane 4: HeLa Cell Lysates; Lane 5: PC-12 Cell Lysates; probed with PSMA5 (426CT8.5.1) Monoclonal Antibody, unconjugated (bsm-51389M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PSMA5 (426CT8.5.1) Monoclonal Antibody (bsm-51389M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51389M

Applications: WB, IHC-P, IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51394M

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: PC-12 probed with TOP1 (1C6) Monoclonal Antibody, unconjugated (bsm-51396M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells stained with TOP1 (1C6) Monoclonal Antibody, bsm-51396M at 1:25 dilution, followed by Alexa Fluor\u00ae 488 goat anti-mouse lgG (green), compared to mouse IgG1 isotype control (blue).
Lane 1: Molt-4 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Siha cell lysates; Lane 4: K562 cell lysates probed with TOP1 (1C6) Monoclonal Antibody, Unconjugated (bsm-51396M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51396M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Daudi Cell lysates; Lane 2: Raji Cell lysates; probed with BCL10 (5C2) Monoclonal Antibody, unconjugated (bsm-51398M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with BCL10 (5C2) Monoclonal Antibody (bsm-51398M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Hela cells probed with using BCL10 (5C2) Monoclonal Antibody (green) unconjugated bsm-51398M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared to an isotype control of mouse IgG (blue).
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with BCL10 (5C2) Monoclonal Antibody, Unconjugated (bsm-51398M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51398M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51399M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Sample Tissue\/Cells lysates probed with PPARA (7G5) Monoclonal Antibody, unconjugated (bsm-51405M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of Hela cells using bsm-51405M PPARA (7G5) Antibody (green) compared to an isotype control of mouse IgG1 (blue). bsm-51405M was diluted at 1:25 dilution. An Alexa Fluor\u00ae 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Mouse NIH\/3T3 cell lysates probed with PPARA Monoclonal Antibody, Unconjugated (bsm-51405M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51405M

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Jurkat Cell lysates; Lane 2: NIH\/3T3 Cell lysates; Lane 3: C6 Cell lysates; Lane 4: PC-12 Cell lysates; Lane 5: Hela Cell lysates; probed with RAD51 (1C9) Monoclonal Antibody, unconjugated (bsm-51402M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Raji cell lysates probed with RAD5 Monoclonal Antibody, Unconjugated (bsm-51402M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51402M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51403M

Applications: WB, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51404M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (RPS6 (9F2)) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (RPS6 (9F2)) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n
25 ug total protein per lane of various lysates (see on figure) probed with RPS6 (N-term) monoclonal antibody, unconjugated (bsm-51406M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51406m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51408M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51407M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51409M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51411M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: U-251 MG Cell lysates; probed with FUBP3 (1216CT820.155.150) Monoclonal Antibody, unconjugated (bsm-51416M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells stained with FUBP3 (12C8) Monoclonal Antibody, bsm-51416M at 1:25 dilution, followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). Cytoplasmic actin was counterstained with Alexa Fluor\u00ae 555 conjugated with Phalloidin (red).

Catalog #1: bsm-51416M

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: NCI-H292 Cell lysates; Lane 2: A549 Cell lysates; Lane 3: HepG2 Cell lysates; probed with ALDH2 (138CT22.3.8) Monoclonal Antibody, unconjugated (bsm-51417M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ANTIBODY Monoclonal Antibody (bsm-51417M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51417M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7 Cell lysates; Lane 2: A549 Cell lysates; Lane 3: A431 Cell lysates; probed with CAPN1 (6C5) Monoclonal Antibody, unconjugated (bsm-51419M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CAPN1 (6C5) Monoclonal Antibody (bsm-51419M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Hela cells probed with using CAPN1 (6C5) Monoclonal Antibody bsm-51419M at 1:25 for 60 minutes followed by A488 secondary antibody at 1:400 (green), compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51419M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51421M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51418M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: rat liver lysates; Lane 2: NIH\/3T3 Cell lysates; Lane 3: MCF-7 Cell lysates; Lane 4: mouse liver lysates; Lane 5: C6 Cell lysates;probed with VCP (4C4) Monoclonal Antibody, unconjugated (bsm-51420M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Fluorescent image of U251 cells stained with VCP (4C4) Antibody (bsm-51420M) at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor\u00ae 555 conjugated with Phalloidin (red).

Catalog #1: bsm-51420M

Applications: WB, FCM, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51428M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated