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Primary Antibodies

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Catalog #1: bs-3836R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Others

Conjugate: HRP

Lane 1: mouse heart lysates Lane 2: mouse kidney lysates probed with Anti PABP (Methyl Arg455\/460) Polyclonal Antibody, Unconjugated (bs-3836R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 75kD. Observed band size: 75kD
Antigen: bs-3836P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n

Catalog #1: bs-3836R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-3836R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat, Others

Conjugate: Biotin

Catalog #1: bs-3836R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: Cy3

Catalog #1: bs-3836R-A350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-3836R-A488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-3836R-A555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-3836R-A594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-3836R-A647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-3836R-A680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-3836R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: Cy5

Catalog #1: bs-3836R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: Cy7

Catalog #1: bs-3836R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: FITC

Catalog #1: bs-3836R-A750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-3769R-A488

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-3769R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-3769R-A647

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-3769R-A350

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-3769R-A594

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 594

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (Di Methyl K36) Polyclonal Antibody, Unconjugated (bs-3769R) at 1:400 overnight at 4\u00b0C, DAB staining.
HeLa cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Histone H3 (Di Methyl K36) Antibody(bs-3769R) at 1: 50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), no antibody control control (blue), secondary antibody only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (Di Methyl K36) Polyclonal Antibody, Unconjugated (bs-3769R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (Di Methyl K36) Polyclonal Antibody, Unconjugated (bs-3769R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3769R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3769R-A555

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-3769R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3769R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-3769R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-3769R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-3769R-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-3769R-PE

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-3769R-A680

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-3769R-A750

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-3773R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3773R-A555

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-3773R-A488

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 488

Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-Histone H3 (Di Methyl K9) Polyclonal Antibody, Unconjugated (bs-3772R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
HeLa cells were fixed with 70% ice-cold methanol overnight at 4\u2103\uff0cpermeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Histone H3 (Di Methyl K9) Antibody(bs-3772R) at 1\uff1a50dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), no antibody control control (blue), secondary antibody only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (Di Methyl K9) Polyclonal Antibody, Unconjugated (bs-3772R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (Di Methyl K9) Polyclonal Antibody, Unconjugated (bs-3772R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3772R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3773R-A350

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-3773R-A647

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-3773R-A594

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-3773R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-3773R-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-3773R-FITC

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-3773R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-3773R-A680

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-3773R-PE

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bs-3773R-A750

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: ALEXA FLUOR® 750

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (Tri Methyl K4) Polyclonal Antibody, Unconjugated (bs-4715R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (Tri Methyl K4) Polyclonal Antibody, Unconjugated (bs-4715R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (Tri Methyl K4) Polyclonal Antibody, Unconjugated (bs-4715R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (Tri Methyl K4) Polyclonal Antibody, Unconjugated (bs-4715R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-4715R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33093M-A350

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 350

Catalog #1: bsm-33093M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33093M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Lane 1: Hela lysates; Lane 2: NIH3T3 lysates probed with Histone H3 (di methyl K79) Polyclonal Antibody, Unconjugated (bs-33093M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (di methyl K79) Monoclonal Antibody, Unconjugated (bsm-33093M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (di methyl K79) Monoclonal Antibody, Unconjugated (bsm-33093M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (di methyl K79) Monoclonal Antibody, Unconjugated (bsm-33093M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33093M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated