| Overview |
| bs-70117R |
| Gephyrin Antibody |
| WB |
| Specific for endogenous levels of the ~93 kDa gephyrin protein in western blots of brain tissue. |
| Human, Rat |
| Specifications |
| Unconjugated |
| Rabbit |
| Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat gephyrin, conjugated to keyhole limpet hemocyanin (KLH). |
| Polyclonal |
| IgG |
| Lot Dependent |
| Antigen Affinity purification from whole antiserum |
| 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| Q03555 |
| Domain E antibody, Domain G antibody, GEPH antibody, GEPH_HUMAN antibody, GPH antibody, GPHN antibody, GPHRYN antibody, KIAA1385 antibody, Molybdopterin molybdenumtransferase antibody, MPT adenylyltransferase antibody, MPT Mo-transferase antibody |
| In neuronal tissue, gephyrin is a scaffolding protein that self assembles in a complex, flat submembraneous lattice that inhibits mobility of the glycine receptors (GlyR) and GABA-A receptors (GABA-A-R) causing clustering at post synaptic sites (Groeneweg et al, 2018). In non-neuronal tissue gephyrin plays a critical role in the molybdendum cofactor (MoCo) biosynthesis of essential life molybdoenzymes, like sulphite oxidase (Groeneweg et al, 2018). Three functional domains have been identified in gephyrin: the stable, structural G and E domains, and the C domain which is intrinsically unstructured leading to multiple isoforms (108, 105, 102, 98, 90 kDa) (Kawasaki, et al 1997). The 93 kDa protein predominantly expressed in the brain and located in the plasma membrane, has a 10X stronger affinity for the GlyR than the GABA-A-R. Gephyrin’s flexibility to change its size and molecular density is directly correlated to its high affinity to the GlyR-β subunit, and is required for anchoring and accurate clustering of GlyRs at post synaptic sites and microtubule transport chains (Greoneweg et al, 2018). A consistent parameter in the pathogenesis of Alzheimers Disease shows a decrease of inhibitory GABAergic synapses and gephyrin, and increased levels of an insoluble 37 kDa gephyrin fragment not detected in healthy, non-AD models (Kiss et al, 2016). |
| Application Dilution |
| WB |
1:300-5000 |
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