HACE1 Monoclonal Antibody

$Lane 1: 293 cell lysates; Lane 2: 293T\/17 cell lysates probed with HACE1 Monoclonal Antibody, Unconjugated (bsm-51691M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: 293 cell lysates; Lane 2: 293T\/17 cell lysates probed with HACE1 Monoclonal Antibody, Unconjugated (bsm-51691M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51691m
Product Name	HACE1 Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human HACE1.
Clonality	Monoclonal
Clone #	#REF!
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	57531
Swiss Prot	Q8IYU2
Subcellular location	Cytoplasm
Synonyms	E3 ubiquitin-protein ligase HACE1; HACE 1; HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; KIAA1320; HACE1_HUMAN.
Background	E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.
Application Dilution
WB	=1:500-1000