Claudin 18 Monoclonal Antibody

$Tissue: Human stomach Section type: Formalin-fixed & Paraffin-embedded\r\nsection Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room\r\ntemperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP\r\n(Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60637R$

Tissue: Human stomach Section type: Formalin-fixed & Paraffin-embedded\r\nsection Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room\r\ntemperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP\r\n(Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60637R

$Tissue: Human gastric carcinoma Section type: Formalin-fixed & Paraffin-embedded\r\nsection Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab\r\ndilution: 1:1000 Primary Ab incubation condition: 1 hour at room\r\ntemperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP\r\n(Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60637R$

Tissue: Human gastric carcinoma Section type: Formalin-fixed & Paraffin-embedded\r\nsection Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab\r\ndilution: 1:1000 Primary Ab incubation condition: 1 hour at room\r\ntemperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP\r\n(Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60637R

$Tissue: Mouse stomach Section type: Formalin-fixed & Paraffin-embedded\r\nsection Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab\r\ndilution: 1:1000 Primary Ab incubation condition: 1 hour at room\r\ntemperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP\r\n(Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60637R$

Tissue: Mouse stomach Section type: Formalin-fixed & Paraffin-embedded\r\nsection Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab\r\ndilution: 1:1000 Primary Ab incubation condition: 1 hour at room\r\ntemperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP\r\n(Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60637R

$Tissue: Rat stomach Section type: Formalin-fixed & Paraffin-embedded\r\nsection Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab\r\ndilution: 1:1000 Primary Ab incubation condition: 1 hour at room\r\ntemperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP\r\n(Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60637R$

Tissue: Rat stomach Section type: Formalin-fixed & Paraffin-embedded\r\nsection Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab\r\ndilution: 1:1000 Primary Ab incubation condition: 1 hour at room\r\ntemperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP\r\n(Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60637R

$Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Claudin 18) Monoclonal Antibody, Unconjugated (bsm-60637R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Claudin 18) Monoclonal Antibody, Unconjugated (bsm-60637R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

IHC-P

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60637R
Product Name	Claudin 18 Monoclonal Antibody
Applications	IHC-P
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Claudin 18
Clonality	Monoclonal
Clone #	R7G12
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	51208
Swiss Prot	P56856
Subcellular location	Cell membrane
Synonyms	Claudin-18; CLD18_HUMAN; CLDN18; SFTA5; SFTPJ.
Application Dilution
IHC-P	1:200-400

Applications

Predicted Reactivity

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