phospho-FADD (Ser194) Recombinant Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: (-): HeLa, (+): HeLa+nocodazole \r\n(500ng\/mL,16 h)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 23 kDa\r\nObserved MW: 23 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: (-): HeLa, (+): HeLa+nocodazole \r\n(500ng\/mL,16 h)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 23 kDa\r\nObserved MW: 23 kDa

Applications

Reactivity

Human

Overview
Catalog #	bsm-60717r
Product Name	phospho-FADD (Ser194) Recombinant Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human phospho-FADD (Ser194)
Modification Site	Ser194
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8772
Swiss Prot	Q13158
Subcellular location	Cytoplasm
Synonyms	FADD_HUMAN; FAS-associated death domain protein; FAS-associating death domain-containing protein; MORT1; GIG3; GIG 3; IMD90; FAS-associating death domain-containing protein; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity;
Application Dilution
WB	1:300-5000