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Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Annexin A13 Polyclonal Antibody, Unconjugated (bs-0634R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti AQP4 Polyclonal Antibody, Unconjugated (bs-0634R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat spinal cord tissue labeled with Anti-AQP4 Polyclonal Antibody, Unconjugated (bs-0634R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Rat Lung lysates probed with Anti- AQP4 Polyclonal Antibody, Unconjugated (bs-0634R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C. Predicted band 35kD. Observed band size: 33kD.

Catalog #1: bs-0634R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-PAR-1 Polyclonal Antibody (bs-0828R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat heart, L2 rat brain lysates probed (bs-0828R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 42kDa.\\n
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Thrombin receptor\/ PAR1) polyclonal Antibody, Unconjugated (bs-0828R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0828R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat carotid artery Anti-MMP-2 Polyclonal Antibody (bs-0412R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated bs-0412R at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MMP2 Polyclonal Antibody, Unconjugated (bs-0412R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mmp2 Polyclonal Antibody, Unconjugated (bs-0412R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0412R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse liver labeled with Rabbit Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat liver lysates L2 mouse intestine lysates probed with Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 25kD. Observed band size:25kD.
Mouse heart lysates probed with Rabbit Anti-Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0471R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse embryo lysates Lane 2: mouse lung lysates probed with Anti SOX2 Polyclonal Antibody, Unconjugated (bs-0523R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 32kD. Observed band size: 32kD.
NCCIT cells probed with Rabbit Anti-SOX2 Polyclonal Antibody (bs-0523R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Paraformaldehyde-fixed, paraffin embedded Mouse embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SOX2 Polyclonal Antibody, Unconjugated (bs-0523R) at 1:400 overnight at 4\u00b0C, DAB staining.
U251 cells (black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SOX2 Antibody(bs-0523R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0523R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical carcinoma tissue labeled with Anti-SynCAM\/TSLC1 Polyclonal Antibody, Unconjugated (bs-1147R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-SynCAM\/TSLC1 Polyclonal Antibody, Unconjugated (bs-1147R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
L1 rat brain lysates, L2 rat heart lysates probed (bs-1147R) Anti-SynCAM\/TSLC1 Polyclonal, Unconjugated at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 44kD.

Catalog #1: bs-1147R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

U-937 cells were stained with CD24 Polyclonal Antibody(bs-4891R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Bone tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Cerebrum tissue lysates probed with CD24 Polyclonal Antibody, Unconjugated (bs-4891R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4891R

Applications: WB, ELISA, FCM, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Nitrotyrosine Polyclonal Antibody, Unconjugated (bs-8551R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Hela probed with Nitrotyrosine Polyclonal Antibody, Unconjugated (bs-8551R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Cpnjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-8551R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Species independent

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast cancer labeled with Anti-CA153\/MUC1\/KL-6 Polyclonal Antibody, Unconjugated (bs-1497R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
MCF7 (Positive) and HL-60 (Negative control) cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MUC1 Antibody (bs-1497R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF-7 cells were stained with MUC1 Polyclonal Antibody, Unconjugated(bs-1497R) at 1:100 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MUC1 Polyclonal Antibody, Unconjugated (bs-1497R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1497R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded bone of mouse embryo labeled with Anti-CATSPER Polyclonal Antibody, Unconjugated (bs-2986R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Aggrecan Polyclonal Antibody, Unconjugated (bs-11655R)at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACAN) Polyclonal Antibody, Unconjugated (bs-11655R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACAN) Polyclonal Antibody, Unconjugated (bs-11655R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-11655R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreatic tissue labeled with Anti-BSEP\/ABCB11 Polyclonal Antibody, Unconjugated (bs-1954R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BSEP ) Polyclonal Antibody, Unconjugated (bs-1954R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BSEP ) Polyclonal Antibody, Unconjugated (bs-1954R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1954R

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Rat

Conjugate: Unconjugated

Mouse brain lysate probed with Anti GALR2 Polyclonal Antibody, Unconjugated (bs-11527R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 42kD. Observed band size: 42kD
Rat H9C2 cells probed with Rabbit Anti-GALR2 Polyclonal Antibody, Unconjugated (bs-11527R) at 6ug for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-11527R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human stomach labeled with Anti-Inhibin beta A\/Activin A Polyclonal Antibody, Unconjugated (bs-1774R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Activin A, Unconjugated (bs-1774R) followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1774R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human ovary carcinoma labeled with Anti-Stra8, Unconjugated (bs-1903R) at 1:800, followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37\u00b0C for 30min; Antibody incubation with Stra8 Polyclonal Antibody, Unconjugated bs-1903R at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Mouse testis lysates probed with Stra8 Polyclonal Antibody, Unconjugated (bs-1903R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-1903R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart labeled with Anti-Nkx2.5\/Cardiac-specific homeobox 1 Polyclonal Antibody, Unconjugated (bs-2054R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Nkx2.5 Polyclonal Antibody, Unconjugated (bs-2054R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2054R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse liver labeled with Anti-PPARGC1A\/PGC1 alpha Polyclonal Antibody, Unconjugated (bs-1832R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin-embedded rat kidney labeled with Anti-PPARGC1A\/PGC1 alpha Polyclonal Antibody, Unconjugated(bs-1832R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
L1 rat brain lysates L2 rat kidney lysates probed with Anti PPARGC1A\/PGC1 alpha Polyclonal Antibody, Unconjugated (bs-1832R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 88kD. Observed band size:88kD.\\n
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PGC1 alpha Polyclonal Antibody, Unconjugated (bs-1832R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1832R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Hela (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CLEC 4E Antibody(bs-8541R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n
THP-1 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CLEC 4E Polyclonal Antibody(bs-8541R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-8541R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-CD19 Polyclonal Antibody, Unconjugated(bs-4755R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy5 conjugated(bs-0295G-Cy5)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Mouse lung and liver lysates probed with CD19 Polyclonal Antibody, unconjugated (bs-4755R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n
Human HL60 Cells were fixed with 70% methanol (Overnight at 4\u2103). The cells were then incubated in 1 X PBS\/2%BSA\/10% goat serum to block non-specific protein-protein interactions followed by incubation with CD19 Polyclonal Antibody, Unconjugated (bs-4755R) at 0.2 \u03bcg\/10^6 cells for 30 minutes at room temp. A PE-conjugated secondary antibody was used for 40 min at room temperature. The graph compares the primary antibody (green) to unstained cells (dark blue), secondary only (light blue), and isotype control (orange).

Catalog #1: bs-4755R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

HeLa lysates probed with Rabbit Anti-Claudin 1 Polyclonal Antibody (bs-1428R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Claudin 1 Polyclonal Antibody, Unconjugated (bs-1428R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Human A549 cell lysates; Lane 2: Human HepG2 cell lysates probed with Claudin 1 Polyclonal Antibody, Unconjugated (bs-1428R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1428R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse small intestine labeled with Rabbit Anti S100A4 Polyclonal Antibody, Unconjugated (bs-3759R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti S100A4 Polyclonal Antibody, Unconjugated (bs-3759R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat skin labeled with Anti-S100A4 Polyclonal Antibody, Unconjugated (bs-3759R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung cancer labeled with Rabbit Anti-S100A4 Polyclonal Antibody, Unconjugated(bs-3759R) 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-3759R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Aggrecanase-2\/ADAMTS5 Polyclonal Antibody, Unconjugated (bs-3573R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Human U87MG cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human HeLa cell lysates probed with ADAMTS5 Polyclonal Antibody, Unconjugated (bs-3573R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3573R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Rabbit Anti CD138\/Syndecan-1 Polyclonal Antibody, Unconjugated (bs-1309R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 human colon carcinoma lysates probed with Anti- CD138\/Syndecan-1 Polyclonal Antibody, Unconjugated (bs-1309R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 34kD.Observed band 34D, 92kD
Paraformaldehyde-fixed, paraffin embedded rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \r\nSyndecan 1 Polyclonal Antibody, Unconjugated (bs-1309R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and for 20 minutes and DAB staining.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Human U251 cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human HeLa cell lysates probed with Syndecan-1 Polyclonal Antibody, Unconjugated (bs-1309R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1309R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin-embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD105 Polyclonal Antibody, Unconjugated (bs-0579R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-0579R\r\n ) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-0579R\r\n ) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-0579R\r\n ) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0579r-TR

Applications: IHC-P, IHC-F, IF

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human nasopharyngeal carcinoma labeled with Anti-Trk-B Polyclonal Antibody (bs-0175R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 rat kidney lysates probed with Anti Trk B\/NTRK2 Polyclonal Antibody, Unconjugated (bs-0175R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 90kD. Observed band size:90kD.\\n
RSC96 cells probed with TrkB Polyclonal Antibody, Unconjugated (bs-0175R) at [CNC.] for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human U251 cell lysates; Lane 3: Human U-87MG cell lysates probed with TrkB Polyclonal Antibody, Unconjugated (bs-0175R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0175R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Rat cerebrum lysates probed with PACAP-27\/38 Polyclonal Antibody, Unconjugated (bs-0190R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat cerebrum lysates probed with PACAP-27\/38 Polyclonal Antibody, Unconjugated (bs-0190R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0190R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD203c Polyclonal Antibody, Unconjugated (bs-1568R) followed by conjugation to the secondary antibody and DAB staining
Human HeLA cells probed with Rabbit Anti-ENPP3\/CD203c Polyclonal Antibody, Unconjugated (bs-1568R) (green) at 1:10 for 30 minutes followed by a FITC conjugated secondary antibody compared to control cells (blue).
Raji (Positive) and Molt-4 (Negative control) cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ENPP3\/CD203c Antibody (bs-1568R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1568R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-1086R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

L1 rat brain lysates L2 rat kidney lysates probed with Anti IL-1R1\/CD121a\/IL1 Receptor I Polyclonal Antibody, Unconjugated (bs-2594R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 62kD. Observed band size:62kD.
MCF7 cells(black) were fixed with 70% ice-cold methanol overnight at 4\u2103,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL1 Receptor I Polyclonal Antibody(bs-2594R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2594R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human endometrium tissue labeled with Anti-KRAS Polyclonal Antibody (bs-1033R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with KRAS Polyclonal Antibody, Unconjugated (bs-1033R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (Goat Anti-rabbit IgG\/Bio) for 20minutes at 37\u00b0C, followed by a conjugated streptavidin at 1:500 for 40 minutes and DAPI staining of the nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with KRAS Polyclonal Antibody, Unconjugated (bs-1033R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Mouse NIH\/3T3 lysates probed with KRAS Polyclonal Antibody, Unconjugated (bs-1033R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1033R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti LBT4-R1 Polyclonal Antibody, Unconjugated (bs-2654R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 39kD. Observed band size: 39kD.
Formalin-fixed and paraffin-embedded liver of mouse embryo labeled with Anti-LTB4-R1\/BLTR Polyclonal Antibody, Unconjugated(bs-2654R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Alexa Fluor 647 conjugated(bs-0295G-A647)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-2654R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Antigen: bs-0292P, BSA, 1ug\/100ul \\nPrimary: bs-0292R, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm. \\n
Lane 1: Human Serum lysates; Lane 2: Mouse Serum lysates; Lane 3: Rat Serum lysates; Lane 4: Goat Serum lysates; Lane 5: Cow Serum lysates probed with BSA Polyclonal Antibody, Unconjugated (bs-0292R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0292R

Applications: WB, ELISA, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat, Cow

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti AIM2 Polyclonal Antibody, Unconjugated (bs-5986R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AIM2 Polyclonal Antibody, Unconjugated (bs-5986R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AIM2 Polyclonal Antibody, Unconjugated (bs-5986R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AIM2 Polyclonal Antibody, Unconjugated (bs-5986R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5986R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti Tissue factor\/CD142\/F3 Polyclonal Antibody, Unconjugated (bs-4690RR) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: rat brain lysates Lane 2: rat liver lysates probed with Anti Tissue factor\/CD142\/F3 Polyclonal Antibody, Unconjugated (bs-4690R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band
Image provided by the Independent Validation Program,badge number 029589:Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD142 Polyclonal Antibody, Unconjugated (bs-4690R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Human A431 cell lysates; Lane 4: Human MDA-MB-231 cell lysates; Lane 5: Human SiHa cell lysates probed with Tissue factor Polyclonal Antibody, Unconjugated (bs-4690R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4690R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Smad2(Ser465 + Ser467) Polyclonal Antibody, Unconjugated (bs-3419R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Placenta lysates; Lane 2: Mouse RAW264.7 cell lysates; Lane 3: Rat Testis lysates; Lane 4: Human Hela cell lysates; Lane 5: Human HT1080 cell lysates; Lane 6: Human Jurkat cell lysates; Lane 7: Human HL-60 cell lysates probed with Smad2 Polyclonal Antibody, Unconjugated (bs-3419R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-Smad2 (Ser465 + Ser467) ) polyclonal Antibody, Unconjugated (bs-3419R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-Smad2 (Ser465 + Ser467)Antibody(bs-3419R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-3419R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200, followed by conjugation to the secondary antibody and DAB staining\\n
This image has been kindly submitted by Efi Tsouko from the University of Houston.\\n\\nHuman LNCaP prostate cancer cells stained with Rabbit Anti-GLUT12 Polyclonal Antibody (bs-2540R)for 30 minutes at 4\u00b0C.Followed by secondary antibody AlexaFluor488 incubation for 30min 4\u00b0C in the dark.\\n
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLUT12 Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2540R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-MAP2\/MAP-2A.B.C Polyclonal Antibody, Unconjugated (bs-1369R) followed by conjugation to the secondary antibody and DAPI staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-MAP2\/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MAP2\/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MAP2\/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1369R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-5598R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-13534R

Applications: ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-4834R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: PE

Formalin-fixed and rat lung tissue labeled with Anti-PTAFR Polyclonal Antibody, Unconjugated (bs-1478R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti-PTAFR Polyclonal Antibody, Unconjugated (bs-1478R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-PTAFR Polyclonal Antibody, Unconjugated (bs-1478R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat lung lysates, L2 rat brain lysates probed (bs-1478R) Anti-PTAFR Polyclonal, Unconjugated at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band siz

Catalog #1: bs-1478R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti PARP (N-Terminus) Polyclonal Antibody, Unconjugated(bs-2138R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI
Lane 1: Rat Brain; Probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:1000 in 4˚C.
Human HeLa lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Human Raji lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-2138R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BNP Polyclonal Antibody, Unconjugated (bs-2207R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-BNP Polyclonal Antibody, Unconjugated(bs-2207R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-2207R

Applications: ELISA, IHC-P, IF(IHC-P)

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded: Human nasopharyngeal carcinoma labeled with Rabbit Anti-BAFF\/CD257 Polyclonal Antibody, Unconjugated (bs-2431R) 1:400 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded: rat liver tissue labeled with Rabbit Anti-BAFF\/CD257 Polyclonal Antibody, Unconjugated (bs-2431R) 1:200 followed by conjugation to the secondary Cy3 antibody
Formalin-fixed and paraffin-embedded: rat liver tissue labeled with Rabbit Anti-BAFF\/CD257 Polyclonal Antibody, Unconjugated (bs-2431R) 1:200 followed by conjugation to the secondary Cy3 antibody

Catalog #1: bs-2431R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti Phospho-TAK1(Thr184\/187) Polyclonal Antibody, Unconjugated (bs-3439R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Phospho-TAK1(Thr184\/187) Polyclonal Antibody, Unconjugated(bs-3439R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-3439R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse kidney lysates probed with Rabbit Anti-ETL\/ELTD1 Polyclonal Antibody, Unconjugated (bs-13111R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue).
Paraformaldehyde-fixed, paraffin embedded rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with ETL\/ELTD1 Polyclonal Antibody, Unconjugated (bs-13111R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tETL\/ELTD1 Polyclonal Antibody, Unconjugated (bs-13111R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining.

Catalog #1: bs-13111R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Mouse stomach lysates probed with SPHK2 Polyclonal Antibody, unconjugated (bs-2653R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Tissue\/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with SPHK2 Polyclonal Antibody, Unconjugated (bs-2653R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37\u00b0C for 90 minutes, DAPI (5ug\/ml, blue) was used to stain the cell nuclei.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (SPHK2) polyclonal Antibody, Unconjugated (bs-2653R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2653r

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Antigen: bs-4572P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti VEGF-A Polyclonal Antibody, Unconjugated (bs-4572R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with VEGF-A Polyclonal Antibody(bs-4572R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-4572R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, GuineaPig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-SDF-1\/CXCL12 Polyclonal Antibody, Unconjugated (bs-4938R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Liver tissue lysates; Lane 2: Human HT-1080 tissue lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human HepG2 cell lysates probed with CXCL12 Polyclonal Antibody, Unconjugated (bs-4938R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4938R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated