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Recombinant Antibodies

Recombinant Antibodies


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Flow cytometric analysis of HepG2 cells with DUSP1 (4G7) Monoclonal Antibody (bsm-52770R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Mouse spleen Probed with DUSP1 (4G7) Monoclonal Antibody (bsm-52770R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with DUSP1 (4G7) Monoclonal Antibody (bsm-52770R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with DUSP1 (4G7) Monoclonal Antibody (bsm-52770R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52770r

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Rat brain cells lysates probed with NUR77 (1C1) Monoclonal Antibody (bsm-52972R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with NUR77 (1C1) Monoclonal Antibody (bsm-52972R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with NUR77 (1C1) Monoclonal Antibody (bsm-52972R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with NUR77 (1C1) Monoclonal Antibody (bsm-52972R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52972r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (bsm-54108R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (bsm-54108R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (bsm-54108R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Ferritin Heavy Chain Monoclonal Antibody, Unconjugated (bsm-54108R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54108r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52309r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human U251 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human 293T cell lysates probed with beta Tubulin Monoclonal Antibody, Unconjugated (bsm-52290R) at 1:20000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated(bsm-52290R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated(bsm-52290R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated(bsm-52290R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-52290r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RAF1 (Ser259) Monoclonal Antibody, Unconjugated (bsm-52194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RAF1 (Ser259) Monoclonal Antibody, Unconjugated (bsm-52194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RAF1 (Ser259) Monoclonal Antibody, Unconjugated (bsm-52194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RAF1 (Ser259) Monoclonal Antibody, Unconjugated (bsm-52194R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52194r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: F9; Lane 2: NCCIT; Lane 3: hES lysates probed with Oct 4 (2D5) Monoclonal Antibody (bsm-52002R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
N2A cells were stained with Oct 4 (2D5) Monoclonal Antibody (bsm-52002R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Western blot analysis of Oct4 on F9 cell lysates with Rabbit anti-Oct4 antibody (bsm-52002R) at 1\/1,000 dilution.\r\n\r\nLysates\/proteins at 10 \u788cg\/Lane.\r\n\r\nPredicted band size: 39 kDa\r\nObserved band size: 45 kDa\r\n\r\nExposure time: 2 minutes;\r\n\r\n12% SDS-PAGE gel.\r\n\r\nProteins were transferred to a PVDF membrane and blocked with 5% NFDM\/TBST for 1 hour at room temperature. The primary antibody (bsm-52002R) at 1\/1,000 dilution was used in 5% NFDM\/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.\r\n
Western blot analysis of Oct4 on different lysates with Rabbit anti-Oct4 antibody (bsm-52002R) at 1\/500 dilution.\r\n\r\nLane 1: HES cell lysate\r\nLane 2: NCCIT cell lysate\r\n\r\nLysates\/proteins at 10 \u00b5g\/Lane.\r\n\r\nPredicted band size: 39 kDa\r\nObserved band size: 45 kDa\r\n\r\nExposure time: 1 minute;\r\n\r\n10% SDS-PAGE gel.\r\n\r\nProteins were transferred to a PVDF membrane and blocked with 5% NFDM\/TBST for 1 hour at room temperature. The primary antibody (bsm-52002R) at 1\/500 dilution was used in 5% NFDM\/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.\r\n

Catalog #1: bsm-52002r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: room temperature 2h\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: 1: MCF-7, 2: T47D, 3: MDA-MB-231\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 15 kDa\r\nObserved MW: 15 kDa\r\n
Cell line: HepG2\r\nFixative: 4% Paraformaldehydel\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary Ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary Ab: Goat Anti-Mouse IgG\r\nNuclear counter stain: DAPI (Blue)\r\nComment: Color green is the positive signal for bsm-60551M
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (FIS1) monoclonal Antibody, Unconjugated (bsm-60551M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.\u7f16\u53f7

Catalog #1: bsm-60551m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated