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Glucocorticoid receptor Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with Glucocorticoid receptor monoclonal antibody, unconjugated (bsm-55727R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Glucocorticoid receptor monoclonal antibody, unconjugated (bsm-55727R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucocorticoid receptor Monoclonal Antibody, Unconjugated (bsm-55727R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-F
  • IF
  • IHC-P

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-55727r-100uL
Product Name Glucocorticoid receptor Recombinant Antibody
Applications
WB ELISA IHC-F IF IHC-P
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Clonality Recombinant
Clone # 12F5
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2908
Swiss Prot P04150
Subcellular location Cytoplasm, Nucleus
Synonyms GCR_HUMAN; NR3C1; GR; Nuclear receptor subfamily 3 group C member 1; GRL; GCCR; GCR; GCRST; glucocorticoid receptor; nuclear receptor subfamily 3, group C, member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Application Dilution
WB =1:500-2000
ELISA ELISA=1:5000-10000
IHC-F IHC-F=1:500-2000
IF IF=1:100-500
IHC-P IHC-P=1:500-200

Frequently asked questions

Which applications is Glucocorticoid receptor Recombinant Antibody (bsm-55727R) validated for, and at what dilution?

bsm-55727R is validated for WB (WB=1:500-2000); ELISA (ELISA=1:5000-10000); IHC-F (IHC-F=1:500-2000); IF (IF=1:100-500); IHC-P (IHC-P=1:500-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Glucocorticoid receptor Recombinant Antibody (bsm-55727R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-55727R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Glucocorticoid receptor Recombinant Antibody (bsm-55727R)?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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