Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

Smad2&Smad3 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with Smad2&Smad3 monoclonal antibody, unconjugated (bsm-55747R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Smad2&Smad3 monoclonal antibody, unconjugated (bsm-55747R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Smad2&Smad3 monoclonal antibody, unconjugated (bsm-55747R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Smad2&Smad3 monoclonal antibody, unconjugated (bsm-55747R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Smad2&Smad3 Monoclonal Antibody, Unconjugated (bsm-55747R) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-F
  • IF
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-55747r-100uL
Product Name Smad2&Smad3 Recombinant Antibody
Applications
WB IHC-F IF IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Smad2
Clonality Recombinant
Clone # 12A2
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4087
Swiss Prot Q15796
Subcellular location Cytoplasm, Nucleus
Synonyms CHTD8; JV18; JV18-1; LDS6; MADH2; MADR2; hMAD-2; hSMAD2; 7120426M23Rik; Smad-2; mMad2; SMAD2_HUMAN; SMAD2; MAD homolog 2; Mothers against DPP homolog 2; Mad-related protein 2 (hMAD-2); SMAD family member 2 (SMAD 2 | Smad2 | hSMAD2); SMAD2_MOUSE; Mad-related protein 2 (mMad2); SMAD family member 2 (SMAD 2 | Smad2); SMAD2_RAT; Mad-related protein 2; SMAD family member 2; MAD, mothers against decapentaplegic homolog 2 (Drosophila); SMAD, mothers against DPP homolog 2 (Drosophila)
Application Dilution
WB 1:500-2000
IHC-F 1:200-800
IF 1:200-800
IHC-P 1:200-800

Frequently asked questions

Which applications is Smad2&Smad3 Recombinant Antibody (bsm-55747R) validated for, and at what dilution?

bsm-55747R is validated for WB (1:500-2000); IHC-F (1:200-800); IF (1:200-800); IHC-P (1:200-800). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Smad2&Smad3 Recombinant Antibody (bsm-55747R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-55747R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Smad2&Smad3 Recombinant Antibody (bsm-55747R)?

KLH conjugated synthetic peptide derived from human Smad2

Search our catalog of hundreds of thousands of products

Free sample offer

Try a recombinant sample

20 µL sample

Freewith your paid order

View product details (opens in a new tab)

View cart

US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

Primary antibody added to your cart

Need a secondary antibody?

Product validation image

View product details and validation

Confirm application and cross-reactivity before use. Prices shown are before eligible cart discounts. Shipping and tax are calculated at checkout.

View cart