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ADORA1 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with Adenosine A1-R monoclonal antibody, unconjugated (bsm-63561R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Adenosine A1-R monoclonal antibody, unconjugated (bsm-63561R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADORA1 Monoclonal Antibody, Unconjugated (bsm-63561R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADORA1 Monoclonal Antibody, Unconjugated (bsm-63561R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADORA1 Monoclonal Antibody, Unconjugated (bsm-63561R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADORA1 Monoclonal Antibody, Unconjugated (bsm-63561R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADORA1 Monoclonal Antibody, Unconjugated (bsm-63561R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADORA1 Monoclonal Antibody, Unconjugated (bsm-63561R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADORA1 Monoclonal Antibody, Unconjugated (bsm-63561R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADORA1 Monoclonal Antibody, Unconjugated (bsm-63561R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-F
  • IP
  • IF
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-63561r-100uL
Product Name ADORA1 Recombinant Antibody
Applications
WB ELISA IHC-F IP IF IHC-P
Reactivity Human, Mouse, Rat
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 134
Swiss Prot P30542
Subcellular location Cell membrane
Synonyms RDC7; A1-AR; A1AR; A1R; AA1R; ARA1; Ri; AA1R_BOVIN; ADORA1; AA1R_HUMAN; AA1R_MOUSE; AA1R_RAT; adenosine A1 receptor; Adenosine receptor A1
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-F 1:50-200
IP 1-2ug
IF 1:50-200
IHC-P 1:50-200

Frequently asked questions

Which applications is ADORA1 Recombinant Antibody (bsm-63561R) validated for, and at what dilution?

bsm-63561R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-F (1:50-200); IP (1-2ug); IF (1:50-200); IHC-P (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ADORA1 Recombinant Antibody (bsm-63561R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-63561R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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