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TrkC Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with NTRK3 | Trk-C monoclonal antibody, unconjugated (bsm-63729R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with NTRK3 | Trk-C monoclonal antibody, unconjugated (bsm-63729R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NTRK3 | Trk-C Monoclonal Antibody, Unconjugated (bsm-63729R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-F
  • IP
  • IF
  • IHC-P

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bsm-63729r-100uL
Product Name TrkC Recombinant Antibody
Applications
WB ELISA IHC-F IP IF IHC-P
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4916
Swiss Prot Q16288
Subcellular location Cell membrane
Synonyms GP145-TrkC; TRKC; gp145(trkC); Ntrk3_tv3; NTRK3_HUMAN; NTRK3; GP145-TrkC (Trk-C); Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase; 2.7.10.1; NTRK3_MOUSE; NTRK3_RAT; neurotrophic receptor tyrosine kinase 3; neurotrophic tyrosine kinase, receptor, type 3; NT-3 growth factor receptor; Trk-C
Application Dilution
WB 1:500-2000
ELISA 1:5000-10000
IHC-F 1:50-200
IP 1:50-200
IF 1:50-200
IHC-P 1:50-200

Frequently asked questions

Which applications is TrkC Recombinant Antibody (bsm-63729R) validated for, and at what dilution?

bsm-63729R is validated for WB (1:500-2000); ELISA (1:5000-10000); IHC-F (1:50-200); IP (1:50-200); IF (1:50-200); IHC-P (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TrkC Recombinant Antibody (bsm-63729R) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bsm-63729R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

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