SARS-COV-2 Spike RBD Protein

OVERVIEW
CATALOG # bs-46016P
PRODUCT NAME
SARS-COV-2 Spike RBD Protein
CONJUGATION Unconjugated
SOURCE SARS-COV-2 Spike RBD Protein is expressed from mammalian with a hFc tag at the C-terminal.
It contains Arg319 - Phe541.
EXPRESSION SYSTEM
HEK293
TAG hFc
MOLECULAR WEIGHT The protein has a predicted MW of 51.7kDa. Due to glycosylation, the protein migrates to 55-70KDa based on the Bis-Tris PAGE result.
ENDOTOXIN
Less than 1EU per ug by the LAL method.
PURITY: > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
FORMULATION
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). 5% trehalose is added as a protectant before lyophilization.
STORAGE The product should be stored at -70℃ or -20℃.
RECONSTITUTION
Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
TARGET
ACCESSION
QHD43416.1
BACKGROUND
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.