Phospho-beta Arrestin 1 (Ser412) Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Phospho-beta Arrestin 1 (Ser412) monoclonal antibody, unconjugated (bsm-61196R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Phospho-beta Arrestin 1 (Ser412) monoclonal antibody, unconjugated (bsm-61196R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-61196r-TR
Product Name	Phospho-beta Arrestin 1 (Ser412) Recombinant Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human beta Arrestin 1 around the phosphorylation site of S412
Modification Site	Ser412
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	408
Swiss Prot	P49407
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	ARB1; ARR1; ARRB1; Arrestin 2; Arrestin beta 1.
Background	Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation.
Application Dilution
WB	1:300-5000