Phospho-IRE1 (Ser724) Recombinant Antibody

$Western blot analysis of (1) K562 cell lysate treated with AP; (2) Untreated K562 cell lysate. Using Phospho-IRE1 (Ser724) (bsm-61238R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) K562 cell lysate treated with AP; (2) Untreated K562 cell lysate. Using Phospho-IRE1 (Ser724) (bsm-61238R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

Reactivity

Human

Overview
Catalog #	bsm-61238r
Product Name	Phospho-IRE1 (Ser724) Recombinant Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human IRE1 alpha around the phosphorylation site of S724
Modification Site	Ser724
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2081
Swiss Prot	O75460
Subcellular location	Cytoplasm, Cell membrane
Synonyms	ERN1, ER to nucleus signalling 1, Inositol-requiring enzyme 1, Inositol-requiring protein 1, Ire1-alpha, IRE1a, HIRE1p, IRE1, Inositol-requiring 1, IRE1P.
Background	Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR).
Application Dilution
WB	1:300-5000