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Unconjugated Primary Antibodies



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Catalog #1: bsm-63186R

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with AMPK beta 1 monoclonal antibody, unconjugated (bsm-63175R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-AMPK beta 1 antibody (bsm-63175R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-63175R

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-14727r-100ul

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30109m-apc-cy7-100t

Applications: IF(IHC-P)

Reactivity: Human

Conjugate: APC-Cy7

Lane 1: U937 cell lysates probed with EIF3S2 Polyclonal Antibody, Unconjugated (bs-14544R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with EIF3S2 polyclonal antibody, unconjugated (bs-14544R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-14544r-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-14461r-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-14447r-100ul

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-14413r-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Hela lysates probed with DNAJC2 Polyclonal Antibody, Unconjugated (bs-14386R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DNAJC2 Polyclonal Antibody, Unconjugated (bs-14386R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DNAJC2 Polyclonal Antibody, Unconjugated (bs-14386R) at 1:200 overnight at 4\u00b0C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with DNAJC2 polyclonal antibody, unconjugated (bs-14386R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-14386r-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RKIP\/PBP Polyclonal Antibody, Unconjugated (bs-1436R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (RKIP\/PBP ) Polyclonal Antibody, Unconjugated (bs-1436R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1436r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-CK17 Polyclonal Antibody, Unconjugated (bs-1431R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Recombinant human CK17 protein(bs-42276P) probed with CK17 Polyclonal Antibody, Unconjugated (bs-1431R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-1431r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-14038r-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-E2F4 Polyclonal Antibody, Unconjugated (bs-1399R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Human A431 cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HL-60 cell lysates probed with E2F4 Polyclonal Antibody, Unconjugated (bs-1399R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-1399r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-13976r-100ul

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Testis lysates; Lane 2: Molt-4 cell; Lane3: U2os cell lysates probed with SIRT4 Polyclonal Antibody, Unconjugated (bs-13924R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates probed with CHRAC1 Polyclonal Antibody, Unconjugated (bs-13924R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-13924r-100ul

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-13640r-100ul

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-13740r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded FKBP10 tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-FKBP10 Polyclonal Antibody, Unconjugated (bs-13175R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
MCF-7 Cell lysates probed with FKBP10 Polyclonal Antibody, unconjugated (bs-13175R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
293T Cell lysates probed with FKBP10 Polyclonal Antibody, unconjugated (bs-13175R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Human HeLa cell lysates; Lane 2: Human LO2 cell lysates probed with FKBP10 Polyclonal Antibody, Unconjugated (bs-13175R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-13175r-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung labeled with Rabbit Anti-Epsin 1\/EPN1 Polyclonal Antibody, Unconjugated (bs-13101R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Epsin 1\/EPN1) Polyclonal Antibody, Unconjugated (bs-13101R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Epsin 1\/EPN1) Polyclonal Antibody, Unconjugated (bs-13101R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Epsin 1) polyclonal Antibody, Unconjugated (bs-13101R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-13101r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12940r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12386r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti OSR2 Polyclonal Antibody, Unconjugated (bs-12377R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (OSR1 + OSR2) Polyclonal Antibody, Unconjugated (bs-12377R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-12377r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12314r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12107r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human stomach labeled with Anti-FUT2 Polyclonal Antibody, Unconjugated (bs-12258R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (FUT2) Polyclonal Antibody, Unconjugated (bs-12258R ) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-12258r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12113r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63347r-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-63360r-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-63314r-100ul

Applications: WB, IP, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63312r-100ul

Applications: WB, IHC-P, IHC-F, IP, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-55665r-100ul

Applications: IHC-P, IHC-F, IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-55650r-100ul

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-55642r-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-55629r-100ul

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cells; Lane 2: A549 cells; Lane 3: MCF-7 cells; Probed with Lactate Dehydrogenase (2D9) Monoclonal Antibody (bsm-52434R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of Hela cells with Lactate Dehydrogenase (2D9) Monoclonal Antibody (bsm-52434R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with Lactate Dehydrogenase (2D9) Monoclonal Antibody (bsm-52434R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Lactate Dehydrogenase (2D9) Monoclonal Antibody (bsm-52434R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52434r-100ul

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41915r-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41914m-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33331m-100ul

Applications: IHC-P, IHC-F, IF, ICC/IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-43790r-100ul

Applications: WB

Reactivity: Chikungunya virus

Conjugate: Unconjugated

Catalog #1: bs-42605r-100ul

Applications: WB

Reactivity: M.tuberculosis

Conjugate: Unconjugated

Catalog #1: bs-25914r-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-25448r-100ul

Applications: ICC/IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-25913r-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-11928r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11819r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse colon labeled with Rabbit Anti CTLA-4 Polyclonal Antibody, Unconjugated (bs-1179R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded mouse tumor labeled with Anti-CTLA-4 Polyclonal Antibody, Unconjugated(bs-1179R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.DAPI was used to stain the cell nucleus.
Formalin-fixed and paraffin-embedded human liver cancer labeled with Anti-CTLA-4 Polyclonal Antibody, Unconjugated(bs-1179R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.DAPI was used to stain the cell nucleus.
Lane 1: Mouse Lung tissue lysates; Lane 2: Rat Spleen tissue lysates; Lane 3: Rat Lung tissue lysates probed with CTLA4 Polyclonal Antibody, Unconjugated (bs-1179R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1179r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11794r-100ul

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\talpha + beta Synuclein Antibody, Unconjugated (bs-11787R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
4% Paraformaldehyde-fixed SK-MEL-28 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (alpha + beta Synuclein) polyclonal Antibody, unconjugated (bs-11787R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The SK-MEL-28(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-alpha + beta Synuclein antibody (bs-11787R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bs-11787r-100ul

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated