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Unconjugated Primary Antibodies



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Paraformaldehyde-fixed, paraffin embedded Human prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LITAF Polyclonal Antibody, Unconjugated (bs-55125R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: K562 cell lysates; Lane 2: Hela cell lysates; Lane 3: HepG2 cell lysates; Lane 4: BT-474 cell lysates probed with LITAF Polyclonal Antibody, Unconjugated (bs-55125R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: LITAF knockout (KO) HeLa cell lysates probed with LITAF Polyclonal Antibody, Unconjugated (bs-55125R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55125R

Applications: WB, IHC-P, IP, IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

C6 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (LGALS3 ) polyclonal Antibody, Unconjugated (bs-55123R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (LGALS3 ) polyclonal Antibody, Unconjugated (bs-55123R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (LGALS3) polyclonal Antibody, Unconjugated (bs-55123R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: HT-29 cell lysates; Lane 2: SGC-7901 cell lysates; Lane 3: Hela cell lysates; Lane 4: Mouse Brain lysates; Lane 5: Mouse Kidney lysates probed with LGALS3 Polyclonal Antibody, Unconjugated (bs-55123R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55123R

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55122R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55121R

Applications: WB, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: A549 cell lysates; Lane 2: Mouse Liver lysates probed with IKBKG Polyclonal Antibody, Unconjugated (bs-55110R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: IKBKG knockout (KO) 293T cell lysates probed with IKBKG Polyclonal Antibody, Unconjugated (bs-55110R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55110R

Applications: WB, IP

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ITGA2 Polyclonal Antibody, Unconjugated (bs-55113R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ITGA2 Polyclonal Antibody, Unconjugated (bs-55113R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: ITGA2 knockout (KO) HeLa cell lysates probed with ITGA2 Polyclonal Antibody, Unconjugated (bs-55113R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: HT1080 cell lysates probed with ITGA2 Polyclonal Antibody, Unconjugated (bs-55113R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55113R

Applications: WB, IHC-P, IF(ICC), IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nIRF3 Polyclonal Antibody, Unconjugated (bs-55112R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human esophageal carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nIRF3 Polyclonal Antibody, Unconjugated (bs-55112R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: IRF3 knockout (KO) HeLa cell lysates probed with IRF3 Polyclonal Antibody, Unconjugated (bs-55112R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: Hela cell lysates; Lane 3: Jurkat cell lysates; Lane 4: HepG2 cell lysates probed with IRF3 Polyclonal Antibody, Unconjugated (bs-55112R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55112R

Applications: WB, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

C6 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IFITM3) polyclonal Antibody, Unconjugated (bs-55108R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IFITM3 ) polyclonal Antibody, Unconjugated (bs-55108R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
U2-OScell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IFITM3 ) polyclonal Antibody, Unconjugated (bs-55108R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Hela cell lysates; Lane 2: IFITM3 knockout (KO) HeLa cell lysates probed with IFITM3 Polyclonal Antibody, Unconjugated (bs-55108R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55108R

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cell lysates; Lane 2: IDE knockout (KO) HeLa cell lysates probed with IDE Polyclonal Antibody, Unconjugated (bs-55107R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: DU145 cell lysates; Lane 2: Mouse Liver cell lysates probed with IDE Polyclonal Antibody, Unconjugated (bs-55107R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IDE) polyclonal Antibody, Unconjugated (bs-55107R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55107R

Applications: WB, IHC-P, IF(ICC), IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Jurkat cell lysates; Lane 4: 293T cell lysates; Lane 5: Mouse Spleen lysates; Lane 6: Mouse Thymus lysates probed with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55106R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55106R

Applications: WB, IHC-P, IHC, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55105R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55105R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55105R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HuR \/ ELAVL1 Polyclonal Antibody, Unconjugated (bs-55105R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-55105R

Applications: WB, IHC-P, IF(ICC), IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HSPB1 Polyclonal Antibody, Unconjugated (bs-55104R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat ovarian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HSPB1 Polyclonal Antibody, Unconjugated (bs-55104R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HSPB1 Polyclonal Antibody, Unconjugated (bs-55104R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HSPB1 Polyclonal Antibody, Unconjugated (bs-55104R) at 1:100 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-55104R

Applications: WB, IHC-P, IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55103R

Applications: WB, IHC, IF

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Unconjugated

Lane 1: 293T cell lysates; Lane 2: HPRT1 knockout (KO) 293T cell lysates probed with HPRT1 Polyclonal Antibody, Unconjugated (bs-55102R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MCF-7 lysates; Lane 2: HL-60 cell lysates; Lane 3: SW480 cell lysates; Lane 4: 293T cell lysates; Lane 5: Hela cell lysates; Lane 6: Mouse brain lysates; Lane 7: Mouse Liver lysates probed with HPRT1 Polyclonal Antibody, Unconjugated (bs-55102R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HPRT1 ) polyclonal Antibody, Unconjugated (bs-55102R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55102R

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293T cell lysates; Lane 2: HNRNPA2B1 knockout (KO) 293T cell lysates probed with HNRNPA2B1 Polyclonal Antibody, Unconjugated (bs-55101R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Human BxPC\r\n-3 cell lysates; Lane 3: Human PC-3 cell lysates; Lane 4: Mouse Spleen lysates probed with HNRNPA2B1 Polyclonal Antibody, Unconjugated (bs-55101R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HNRNPA2B1) polyclonal Antibody, Unconjugated (bs-55101R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HNRNPA2B1) polyclonal Antibody, Unconjugated (bs-55101R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55101R

Applications: WB, IHC-P, IF(ICC), IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293T cell lysates; Lane 2: HMGCR knockout (KO) 293T cell lysates probed with HMGCR Polyclonal Antibody, Unconjugated (bs-55100R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: Hela cell lysates probed with HMGCR Polyclonal Antibody, Unconjugated (bs-55100R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55100R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HMGB1 Polyclonal Antibody, Unconjugated (bs-55099R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HMGB1 Polyclonal Antibody, Unconjugated (bs-55099R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HMGB1 Polyclonal Antibody, Unconjugated (bs-55099R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: 293T cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Jurkat cell lysates; Lane 4: Mouse Spleen lysates; Lane 5: Mouse Lung lysates; Lane 6: Mouse kidney lysates; Lane 7: Rat lung lysates probed with HMGB1 Polyclonal Antibody, Unconjugated (bs-55099R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55099R

Applications: WB, IHC-P, IF(ICC), IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cell lysates; Lane 2: HINT1 knockout (KO) HeLa cell lysates probed with HINT1 Polyclonal Antibody, Unconjugated (bs-55097R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 22RV1 cell lysates; Lane 2: Hela cell lysates; Lane 3: H460 cell lysates; Lane 4: K562 cell lysates; Lane 5: Mouse Kidney lysates; Lane 6: Mouse Liver lysates; Lane 7: Rat Kidney lysates probed with HINT1 Polyclonal Antibody, Unconjugated (bs-55097R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55097R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HELLS Polyclonal Antibody, Unconjugated (bs-55095R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: MCF-7 cell lysates; Lane 2: A549 cell lysates; Lane 3: 293T cell lysates; Lane 4: HepG2 cell lysates; Lane 5: Mouse thymus lysates probed with HELLS Polyclonal Antibody, Unconjugated (bs-55095R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: HELLS knockout (KO) HeLa cell lysates probed with HELLS Polyclonal Antibody, Unconjugated (bs-55095R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55095R

Applications: WB, IHC-P, IF(ICC), IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HDAC8 Polyclonal Antibody, Unconjugated (bs-55094R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HDAC8 Polyclonal Antibody, Unconjugated (bs-55094R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HDAC8 Polyclonal Antibody, Unconjugated (bs-55094R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: 293T cell lysates; Lane 2: Mouse Lung lysates probed with HDAC8 Polyclonal Antibody, Unconjugated (bs-55094R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55094R

Applications: WB, IHC-P, IHC, IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293T cell lysates; Lane 2: HADHA knockout (KO) 293T cell lysates probed with HADHA Polyclonal Antibody, Unconjugated (bs-55092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: THP-1 cell lysates; Lane 3: SW480 cell lysates; Lane 4: PC-3 cell lysates; Lane 5: Hela cell lysates; Lane 6: Mouse Intestine lysates; Lane 7: Mouse Heart lysates; Lane 8: Mouse Fat lysates probed with HADHA Polyclonal Antibody, Unconjugated (bs-55092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HADHA) polyclonal Antibody, Unconjugated (bs-55092R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HADHA) polyclonal Antibody, Unconjugated (bs-55092R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55092R

Applications: WB, IF(ICC), IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: DU145 cell lysates; Lane 2: K562 cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Spleen lysates; Lane 6: Mouse Brain lysates; Lane 7: Rat Kidney lysates probed with GSS Polyclonal Antibody, Unconjugated (bs-55091R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: GSS knockout (KO) 293T cell lysates probed with GSS Polyclonal Antibody, Unconjugated (bs-55091R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55091R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: LO2 cell lysates; Lane 2: Hela cell lysates; Lane 3: 22Rv1 cell lysates; Lane 4: U251-MG cell lysates probed with GSK3B Polyclonal Antibody, Unconjugated (bs-55090R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: GSK3B knockout (KO) HeLa cell lysates probed with GSK3B Polyclonal Antibody, Unconjugated (bs-55090R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GSK3B ) polyclonal Antibody, Unconjugated (bs-55090R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55090R

Applications: WB, IF(ICC), IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55088R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GNA13 Polyclonal Antibody, Unconjugated (bs-55086R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GNA13 Polyclonal Antibody, Unconjugated (bs-55086R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: GNA13 knockout (KO) HeLa cell lysates probed with GNA13 Polyclonal Antibody, Unconjugated (bs-55086R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: BT474 cell lysates; Lane 3: Jurkat cell lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Lung lysates; Lane 6: Mouse Spleen lysates; Lane 7: Rat kidney lysates probed with GNA13 Polyclonal Antibody, Unconjugated (bs-55086R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55086R

Applications: WB, IHC-P, IF(ICC), IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: LO2 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: HL60 cell lysates; Lane 4: HT-1080 cell lysates probed with GNAI3 Polyclonal Antibody, Unconjugated (bs-55087R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: GNAI3 knockout (KO) HeLa cell lysates probed with GNAI3 Polyclonal Antibody, Unconjugated (bs-55087R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55087R

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

C6 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GIT1) polyclonal Antibody, Unconjugated (bs-55084R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GIT1) polyclonal Antibody, Unconjugated (bs-55084R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GIT1) polyclonal Antibody, Unconjugated (bs-55084R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: U-251MG cell lysates; Lane 2: A549 cell lysates; Lane 3: A375 cell lysates; Lane 4: Mouse Heart lysates; Lane 5: Rat Testis lysates probed with GIT1 Polyclonal Antibody, Unconjugated (bs-55084R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55084R

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293T cell lysates; Lane 2: GDI2 knockout (KO) 293T cell lysates probed with GDI2 Polyclonal Antibody, Unconjugated (bs-55083R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HT-29 cell lysates; Lane 2: A549 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: U87MG cell lysates; Lane 5: Mouse spleen lysates; Lane 6: Mouse kidney lysates; Lane 7: Mouse lung lysates; Lane 8: Rat liver lysates; Lane 9: Rat spinal cord lysates probed with GDI2 Polyclonal Antibody, Unconjugated (bs-55083R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (GDI2) polyclonal Antibody, Unconjugated (bs-55083R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55083R

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FUS Polyclonal Antibody, Unconjugated (bs-55081R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FUS Polyclonal Antibody, Unconjugated (bs-55081R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FUS Polyclonal Antibody, Unconjugated (bs-55081R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FUS Polyclonal Antibody, Unconjugated (bs-55081R) at 1:100 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-55081R

Applications: WB, IHC-P, IF(ICC), IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55080R

Applications: WB, IHC, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FSCN1 Polyclonal Antibody, Unconjugated (bs-55078R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: FSCN1 knockout (KO) HeLa cell lysates probed with FSCN1 Polyclonal Antibody, Unconjugated (bs-55078R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: U251MG cell lysates; Lane 2: Hela cell lysates; Lane 3: NCI-H460 cell lysates; Lane 4: HepG2 cell lysates; Lane 5: SH-SY5Y cell lysates; Lane 6: Mouse Brain lysates; Lane 7: Mouse spinal cord lysates probed with FSCN1 Polyclonal Antibody, Unconjugated (bs-55078R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat skin cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (FSCN1) polyclonal Antibody, Unconjugated (bs-55078R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55078R

Applications: WB, IHC-P, IF(ICC), IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human H460 cell lysates probed with Fatty Acid Synthase Polyclonal Antibody, Unconjugated (bs-55077R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: Fatty Acid Synthase knockout (KO) 293T cell lysates probed with Fatty Acid Synthase Polyclonal Antibody, Unconjugated (bs-55077R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Fatty Acid Synthase) polyclonal Antibody, Unconjugated (bs-55077R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Fatty Acid Synthase Polyclonal Antibody, Unconjugated (bs-55077R) at 1:100 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-55077R

Applications: WB, IHC-P, IF(ICC), IP, IHC, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-55075R

Applications: WB, IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Tissue\/cell: U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (EZH2 ) polyclonal Antibody, Unconjugated (bs-55072R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: MCF-7 cell lysates; Lane 2: 293T cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Hela cell lysates; Lane 5: SW620 cell lysates; Lane 6: Raji cell lysates; Lane 7: Mouse testis lysates probed with EZH2 Polyclonal Antibody, Unconjugated (bs-55072R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: EZH2 knockout (KO) HeLa cell lysates probed with EZH2 Polyclonal Antibody, Unconjugated (bs-55072R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55072R

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HL-60 cell lysates; Lane 2: THP-1 cell lysates; Lane 3: Hela cell lysates; Lane 4: 293T cell lysates; Lane 5: Mouse testis lysates; Lane 6: Mouse pancreas lysates; Lane 7: Mouse thymus lysates; Lane 8: Mouse spleen lysates probed with ERP29 Polyclonal Antibody, Unconjugated (bs-55071R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: ERP29 knockout (KO) 293T cell lysates probed with ERP29 Polyclonal Antibody, Unconjugated (bs-55071R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55071R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55067R

Applications: WB, IHC, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Human MCF-7 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Mouse NIH\/3T3 cell lysates; Lane 5: Mouse fat lysates; Lane 6: Mouse heart lysates probed with EIF4EBP1 Polyclonal Antibody, Unconjugated (bs-55065R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: EIF4EBP1 knockout (KO) HeLa cell lysates probed with EIF4EBP1 Polyclonal Antibody, Unconjugated (bs-55065R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (EIF4EBP1) polyclonal Antibody, Unconjugated (bs-55065R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55065R

Applications: WB, IF(ICC), IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Eif2ak2 Polyclonal Antibody, Unconjugated (bs-55064R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: Jurkat cell lysates probed with Eif2ak2 Polyclonal Antibody, Unconjugated (bs-55064R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Eif2ak2 ) polyclonal Antibody, Unconjugated (bs-55064R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55064R

Applications: WB, IHC-P, IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EGLN1 Polyclonal Antibody, Unconjugated (bs-55063R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EGLN1 Polyclonal Antibody, Unconjugated (bs-55063R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EGLN1 Polyclonal Antibody, Unconjugated (bs-55063R) at 1:100 overnight at 4\u00b0C, DAB staining.
L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (EGLN1) polyclonal Antibody, Unconjugated (bs-55063R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55063R

Applications: WB, IHC-P, IF(ICC), IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55058R

Applications: WB, IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cell lysates; Lane 2: EEF2K knockout (KO) HeLa cell lysates probed with EEF2K Polyclonal Antibody, Unconjugated (bs-55060R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: Hela cell lysates; Lane 3: Lovo cell lysates; Lane 4: 22Rv1 cell lysates; Lane 5: 293T cell lysates; Lane 6: Mouse Spleen lysates probed with EEF2K Polyclonal Antibody, Unconjugated (bs-55060R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (EEF2K) polyclonal Antibody, Unconjugated (bs-55060R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55060R

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EEF1B2 Polyclonal Antibody, Unconjugated (bs-55059R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EEF1B2 Polyclonal Antibody, Unconjugated (bs-55059R) at 1:100 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EEF1B2 Polyclonal Antibody, Unconjugated (bs-55059R) at 1:100 overnight at 4\u00b0C, DAB staining.
Lane 1: MCF-7 cell lysates; Lane 2: HL-60 cell lysates; Lane 3: 293T cell lysates; Lane 4: Mouse pancreas lysates probed with EEF1B2 Polyclonal Antibody, Unconjugated (bs-55059R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55059R

Applications: WB, IHC-P, IHC, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cell lysates; Lane 2: DPYSL2 knockout (KO) HeLa cell lysates probed with DPYSL2 Polyclonal Antibody, Unconjugated (bs-55057R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: U-251MG cell lysates; Lane 3: 293T cell lysates probed with DPYSL2 Polyclonal Antibody, Unconjugated (bs-55057R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55057R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293T cell lysates; Lane 2: SW480 cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: DNMT1 knockout (KO) 293T cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
293T cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DNMT1) polyclonal Antibody, Unconjugated (bs-55056R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human Jurkat cell lysates; Lane 2: Human Molt-4 cell lysates; Lane 3: Human K562 cell lysates; Lane 4: Human 293T cell lysates ; Lane 5: Human U251 cell lysates ; Lane 6: Human HL60 cell lysates probed with DNMT1 Polyclonal Antibody, Unconjugated (bs-55056R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55056R

Applications: WB, IF(ICC), IP, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293T cell lysates; Lane 2: MCF-7 lysates; Lane 3: BxPC-3 cell lysates; Lane 4: BT-474 cell lysates; Lane 5: SW620 cell lysates; Lane 6: K562 cell lysates; Lane 7: Mouse testis lysates; Lane 8: Mouse spleen lysates; Lane 9: Mouse thymus lysates probed with DNAJB1 Polyclonal Antibody, Unconjugated (bs-55055R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates; Lane 2: DNAJB1 knockout (KO) 293T cell lysates probed with DNAJB1 Polyclonal Antibody, Unconjugated (bs-55055R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DNAJB1) polyclonal Antibody, Unconjugated (bs-55055R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
C6 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DNAJB1) polyclonal Antibody, Unconjugated (bs-55055R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55055R

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human Jurkat cell lysates; Lane 2: Human H-460 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Mouse Brain lysates; Lane 5: Rat Brain lysates probed with DFFA Polyclonal Antibody, Unconjugated (bs-55054R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: DFFA knockout (KO) HeLa cell lysates probed with DFFA Polyclonal Antibody, Unconjugated (bs-55054R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DFFA ) polyclonal Antibody, Unconjugated (bs-55054R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (DFFA ) polyclonal Antibody, Unconjugated (bs-55054R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuc

Catalog #1: bs-55054R

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-55053R

Applications: WB, IP, IHC, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Hela cell lysates; Lane 2: DDIT4 knockout (KO) HeLa cell lysates probed with DDIT4 Polyclonal Antibody, Unconjugated (bs-55052R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: A549 cell lysates; Lane 2: K562 cell lysates; Lane 3: DU145 cell lysates; Lane 4: MCF-7 cell lysates probed with DDIT4 Polyclonal Antibody, Unconjugated (bs-55052R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-55052R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated