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Western Blot Antibodies



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Catalog #1: bsm-33118m-pe-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-33118m-apc-cy7-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-33118m-cy3-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33118m-cy5-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33118m-bf680-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33118m-bf647-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33118m-bf594-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33118m-bf555-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33118m-apc-cy5.5-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-33118m-bf488-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33118m-bf750-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Spleen tissue lysates ; Lane 5: Rat Cerebrum tissue lysates; Lane 6: Human Jurkat cell lysates; Lane 7: Human Raji cell lysates; Lane 8: Human MCF-7 cell lysates; Lane 9: Human A431 cell lysates; Lane 10: Human Hela cell lysates probed with Peroxiredoxin 1 Monoclonal Antibody, Unconjugated (bsm-33118M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Peroxiredoxin 1) polyclonal Antibody, Unconjugated (bsm-33118M) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Peroxiredoxin 1 Antibody(bsm-33118M)at 1:200 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bsm-33118m-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33118m-bf405-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33118m-bf350-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33118m-apc-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-33024m-rbitc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: RBITC

Catalog #1: bsm-33024m-percp-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33024m-fitc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: FITC

Catalog #1: bsm-33024m-percp-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PerCP-Cy7

Catalog #1: bsm-33024m-percp-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PerCP

Catalog #1: bsm-33024m-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy5.5

Catalog #1: bsm-33024m-pe-cy7-100ul

Applications: WB, FCM

Reactivity: Species independent

Conjugate: PE-Cy7

Catalog #1: bsm-33024m-cy5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy5

Catalog #1: bsm-33024m-pe-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PE

Catalog #1: bsm-33024m-pe-Cy5.5-100ul

Applications: WB, FCM

Reactivity: Species independent

Conjugate: PE-Cy5.5

Catalog #1: bsm-33024m-cy3-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy3

Catalog #1: bsm-33024m-pe-cy5-100ul

Applications: WB, FCM

Reactivity: Species independent

Conjugate: PE-Cy5

Catalog #1: bsm-33024m-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy7

Catalog #1: bsm-33024m-bf647-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33024m-bf750-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33024m-bf680-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33024m-bf594-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33024m-bf555-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33024m-bf405-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33024m-bf488-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33024m-bf350-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33024m-apc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: APC

Catalog #1: bsm-33024m-apc-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: APC-Cy5.5

Catalog #1: bsm-33024m-apc-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: APC-Cy7

Catalog #1: bsm-33024m-100ul

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Unconjugated

Lane 1: Human Hela cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human Jurkat cell lysates probed with phospho-Src (Tyr419) Monoclonal Antibody, Unconjugated (bsm-52208R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
The A431 (treated with pervanadate\uff080.5 mM\uff0c30min)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-Src (Tyr419) antibody (bsm-52208R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-52208r-100ul

Applications: WB, Flow-Cyt

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63078r-apc-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-63078r-bf647-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-63077r-percp-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-63078r-bf405-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-63077r-percp-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-63078r-apc-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-63077r-rbitc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC