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Western Blot Antibodies



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Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng; Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng; Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng; Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 2ng ; Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 1ng;Lane 6: SARS-CoV-2 N protein(Cat# bs-41408P) at 0.5ng probed with SARS-CoV-2 N protein monoclonal Antibody, Unconjugated (bsm-41413M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) (bs-41408P); Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) (bs-41451P); Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag) (bs-41452P); Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) (bs-41491P); Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag) (bs-41492P); Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) (bs-41494P) probed with SARS-CoV-2(2019-nCoV)Nucleocapsid Monoclonal Antibody, Unconjugated (bsm-41413M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-41413m-100ul

Applications: WB, ELISA, FCM

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60742m-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30148m-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70350M

Applications: WB, FCM, IP

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A549 Cell lysates; Lane 2: SK-BR-3 Cell lysates; Lane 3: C2C12 Cell lysates; probed with CAPN2 (1C1) Monoclonal Antibody, unconjugated (bsm-51429M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CAPN2 (1C1) Monoclonal Antibody (bsm-51429M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Flow cytometric analysis of U-87 MG cells using bsm-51429M CAPN2 Antibody (green) compared to mouse IgG2b isotype control (blue). bsm-51429M was diluted at 1:100 dilution. Alexa Fluor\u00ae 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Catalog #1: bsm-51429M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MYO18B (3C5) Monoclonal Antibody (bsm-51376M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MYO18B (3C5) Monoclonal Antibody (bsm-51376M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
A431 cells probed with using MYO18B (3C5) Monoclonal Antibody (green) unconjugated bsm-51376M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG2b (blue).
Recombinant protein lysates probed with MYO18B (3C5) Polyclonal Antibody, unconjugated (bsm-51376M) at 1:16000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51376M

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MOLT-4 Cell lysates; probed with ETS1 (1C1) Monoclonal Antibody, unconjugated (bsm-51344M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 cells probed with using ETS1 (1C1) Monoclonal Antibody (green) unconjugated bsm-51344M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared to an isotype control of mouse IgG2b (blue).

Catalog #1: bsm-51344M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: HL-60 Cell lysates; Lane 3: U-2OS Cell lysates; probed with RAB5C (6C1) Monoclonal Antibody, unconjugated (bsm-51343M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
U-2OS cells probed with using RAB5C (6C1) Monoclonal Antibod bsm-51343M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody (green) at 1:400 compared to an isotype control of mouse IgG2b (blue).

Catalog #1: bsm-51343M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with DENR (2C9) Monoclonal Antibody (bsm-51318M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with DENR (2C9) Monoclonal Antibody (bsm-51318M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: A431; Lane 2: human skeletal muscle lysates; Lane 3: human brain Cell lysates; probed with DENR (2C9) Polyclonal Antibody, unconjugated (bsm-51318M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells probed with using DENR (2C9) Monoclonal Antibody bsm-51318M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green), compared to an isotype control of mouse IgG2b (blue).

Catalog #1: bsm-51318M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated