RECEIVE 25% OFF PUBLISHED ANTIBODIES WITH CODE "PUB25"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Western Blot Antibodies



×

Collection Menu

Catalog #1: bsm-41411m-100ug

Applications: WB, ELISA, IHC-P

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41414m-100ul

Applications: WB, ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng; Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng; Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng; Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 2ng ; Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 1ng;Lane 6: SARS-CoV-2 N protein(Cat# bs-41408P) at 0.5ng probed with SARS-CoV-2 N protein monoclonal Antibody, Unconjugated (bsm-41413M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) (bs-41408P); Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) (bs-41451P); Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag) (bs-41452P); Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) (bs-41491P); Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag) (bs-41492P); Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) (bs-41494P) probed with SARS-CoV-2(2019-nCoV)Nucleocapsid Monoclonal Antibody, Unconjugated (bsm-41413M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-41413m-100ul

Applications: WB, ELISA, FCM

Reactivity:

Conjugate: Unconjugated

Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng; Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng; Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng; Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 2ng ; Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 1ng;Lane 6: SARS-CoV-2 N protein(Cat# bs-41408P) at 0.5ng probed with SARS-CoV-2 N protein monoclonal Antibody, Unconjugated (bsm-41412M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) (bs-41408P); Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) (bs-41451P); Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag) (bs-41452P); Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) (bs-41491P); Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag) (bs-41492P); Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) (bs-41494P) probed with SARS-CoV-2(2019-nCoV)Nucleocapsid Monoclonal Antibody, Unconjugated (bsm-41412M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-41412m-100ul

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-41415m-100ul

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-49131m-100ul

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-41411M-HRP

Applications: WB, ELISA, IHC-P

Reactivity: SARS-CoV-2

Conjugate: HRP

Lane 1: A431 Cell lysates; Lane 2: HepG2 Cell lysates; Lane 3: NIH\/3T3 Cell lysates; Lane 4: mouse brain lysates; Lane 5: C6 Cell lysates; Lane 6: PC-12 Cell lysates; probed with YWHAZ (1B6) Monoclonal Antibody, unconjugated (bsm-51397M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YWHAZ (1B6) Monoclonal Antibody, Unconjugated (bsm-51397M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-51397M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-49134m-100ul

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-49133m-100ul

Applications: WB, ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-49132m-100ul

Applications: WB, ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using the H3K4me1 Monoclonal Antibody (bsm-53013M) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the molecular weight marker (in kDa) is shown on the left.
HeLa cells were stained with H3K4me1 (34A1) Monoclonal Antibody (Cat. No. bsm-53013M) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were labelled with the H3K4me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge is shown on the right.
To test the specificity an ELISA was performed using a serial dilution of H3K4me1 (34A1) Monoclonal Antibody (Cat. No. bsm-53013M). The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the monomethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.
ChIP assays were performed using HeLa cells, H3K4me1 (34A1) Monoclonal Antibody (bsm-53013M) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.6 million cells. A titration of the antibody consisting of 1, 5 and 10 \u03bcg per ChIP experiment was analyzed. IgG (5 \u03bcg\/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bsm-53013M

Applications: WB, ELISA, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: K562 Cell lysates; Lane 2: mouse brain lysates; Lane 3: U-251 MG Cell lysates; probed with BAIAP2 (1037CT15.1.3) Monoclonal Antibody, unconjugated (bsm-51410M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Cerebrum lysates probed with BAIAP2 (4G7) Monoclonal Antibody, Unconjugated (bsm-51410M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51410M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51207M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-41663m-pbs-only-100ul

Applications: WB, ELISA

Reactivity: Human

Conjugate: PBS Only

Catalog #1: bsm-41470m-pbs-only-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: PBS Only

Catalog #1: bsm-49133m-percp-cy7-100ul

Applications: WB

Reactivity:

Conjugate: PerCP-Cy7

Catalog #1: bsm-41411m-percp-cy7-100ug

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: PerCP-Cy7

Catalog #1: bsm-60955m-100ul

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60742m-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41470m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-41470m-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-41470m-percp-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-41470m-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-41470m-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-41470m-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-41470m-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-41470m-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-41470m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-41470m-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-41470m-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-41470m-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-41470m-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-41470m-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-41470m-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-41470m-cy5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-41470m-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-41470m-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-41470m-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-41470m-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-41470m-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-41470m-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-41470m-apc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-41470m-apc-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-30148m-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41663m-percp-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-41663m-rbitc-100ul

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-41663m-percp-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP