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Western Blot Antibodies



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Catalog #1: bs-5209r-bf405-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-5209r-cy5-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-5209r-bf488-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-1027r-pbs-only-100l

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: PBS Only

Catalog #1: bs-5209r-bf555-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-5209r-bf750-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-5209r-pe-cy5-100l

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-5209r-bf350-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-5209r-apc-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-5209r-bf680-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-5209r-bf594-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-5209r-pe-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-1027r-percp-cy7-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: PerCP-Cy7

Catalog #1: bs-1027r-percp-cy55-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: PerCP-Cy5.5

Catalog #1: bs-1027r-pe-cy7-100l

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: PE-Cy7

Catalog #1: bs-5209r-bf647-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-phospho-AKT3 (Ser472)Polyclonal Antibody, Unconjugated (bs-5209R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-AKT3 (Ser472)Polyclonal Antibody, Unconjugated (bs-5209R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining
Lane 1: Human HUVEC cell lysates; Lane 2: Human A431 cell lysates probed with AKT1\/2\/3 Polyclonal Antibody, Unconjugated (bs-5209R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1\/2\/3 (Ser472\/Ser473\/Ser474) Polyclonal Antibody, Unconjugated (bs-5209R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5209r-100l

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-5209r-apc-cy55-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-1027r-percp-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: PerCP

Catalog #1: bs-1027r-pe-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: PE

Catalog #1: bs-1027r-rbitc-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: RBITC

Catalog #1: bs-1027r-fitc-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: FITC

Catalog #1: bs-1027r-pe-cy5-100l

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: PE-Cy5

Catalog #1: bs-1027r-pe-cy55-100l

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: PE-Cy5.5

Human A549 probed with Rabbit Anti-CD80 Polyclonal Antibody, Unconjugated (bs-1479R) at 1:300 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.
Lane 1: Mouse RAW264.7 cell lysates; Lane 2: Mouse Sp2\/0 cell lysates; Lane 3: Rat Thymus lysates; Lane 4: Human Raji cell lysates; Lane 5: Human K562 cell lysates probed with CD80 Polyclonal Antibody, Unconjugated (bs-1479R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Raji cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CD80 Polyclonal Antibody(bs-1479R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-1479r-100l

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1027r-bf750-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: AbBy Fluor-750

Catalog #1: bs-1027r-apc-cy7-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: APC-Cy7

Catalog #1: bs-1027r-bf647-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: AbBy Fluor-647

Catalog #1: bs-0091r-bf350-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bs-0091r-cy55-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-0091r-apc-cy7-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy7

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-NR2B\/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: : rat brain tissue labeled with Anti-NR2B\/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) at 1:200 for 40 minutes at 37\u00b0C and DAPI staining
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-NR2B\/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) at 1:200 for 40 minutes at 37\u00b0C
A549 lysates probed with NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37\u02daC.

Catalog #1: bs-0222r-100l

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0091r-apc-cy55-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bs-0091r-percp-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bs-0091r-bf750-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bs-1027r-apc-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: APC

Catalog #1: bs-1027r-cy3-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: Cy3

Catalog #1: bs-1027r-bf555-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: AbBy Fluor-555

Catalog #1: bs-0091r-apc-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: APC

Catalog #1: bs-1027r-bf350-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: AbBy Fluor-350

Catalog #1: bs-1027r-cy7-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: Cy7

Catalog #1: bs-1027r-bf680-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: AbBy Fluor-680

Catalog #1: bs-0091r-bf680-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bs-0091r-rbitc-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bs-0091r-cy3-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-1027r-cy5-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Others

Conjugate: Cy5

Catalog #1: bs-0091r-fitc-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-0091r-cy7-100l

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7