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Western Blot Antibodies



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Catalog #1: bsm-10832m-percp-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-10832m-apc-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-10832m-pe-cy7-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-0295p-pbs-only-100ug

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: PBS Only

Catalog #1: bsm-62518r-pbs-only-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: PBS Only

Catalog #1: bs-6640r-percp-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bs-6640r-pbs-only-100ul

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PBS Only

Catalog #1: bsm-41304m-pbs-only-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: PBS Only

25 ug total protein per lane of various lysates (see on figure) probed with GCSAM monoclonal antibody, unconjugated (bsm-61813R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD163L1 antibody (bsm-61813R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n\r\n
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GCSAM Monoclonal Antibody, Unconjugated (bsm-61813R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GCSAM Monoclonal Antibody, Unconjugated (bsm-61813R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61813r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1\/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ERK1\/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with ERK1\/2 Monoclonal Antibody, Unconjugated (bsm-33232M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-ERK1\/2 antibody (bsm-33232M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-33232m-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated(bsm-33042R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated(bsm-33042R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated(bsm-33042R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Histone H3 Monoclonal Antibody, Unconjugated(bsm-33042R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-33042r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), ChIP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61813r-rbitc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-61813r-pe-cy5-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-61813r-pe-cy7-100ul

Applications: WB, FCM

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-61813r-fitc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-61813r-bf750-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61813r-percp-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-61813r-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-61813r-pe-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-61813r-cy5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-61813r-cy3-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-61813r-bf647-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61813r-apc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-61813r-bf488-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61813r-bf594-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61813r-bf555-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61813r-bf680-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-61813r-bf405-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61813r-apc-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-61813r-bf350-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-52543r-pe-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-52543r-rbitc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-52543r-percp-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-52543r-pe-cy7-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-52543r-fitc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-52543r-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-52543r-pe-cy5-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-52543r-bf594-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-52543r-cy5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-52543r-bf680-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-52543r-cy3-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-52543r-bf750-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-52543r-bf405-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-52543r-bf647-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-52543r-bf350-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-52543r-bf555-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-52543r-bf488-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-52543r-apc-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7