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Lane 1: HL60 cell lysates; Lane 2: Mouse testis lysates probed with RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates;Lane 2: Rat Cerebrum lysates;Lane 3: Mouse heart lysates; Lane 4: Rat heart lysates probed with RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse thyroid; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RUNX1 + RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:400 overnight at 4\u00b0C, DAB staining.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RUNX1 + RUNX2 Polyclonal Antibody(bs-R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1134R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat ovary tissue labeled with Anti-Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-1032R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat brain lysates Lane 2: rat kidney lysates probed with Anti Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-1032R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 39kD. Observed band size: 39kD

Catalog #1: bs-1032R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1083R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human thyroid carcinoma labeled with Anti-SKP2\/skp2 p45 Polyclonal Antibody, Unconjugated (bs-1096R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
MCF-7 cells were stained with SKP2 Polyclonal Antibody, Unconjugated(bs-1096R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Hela cells probed with SKP2 Polyclonal Antibody, Unconjugated (bs-1096R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
293T lysates probed with SKP2 Polyclonal Antibody, Unconjugated (bs-1096R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1096R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart labeled with Anti-WDR26 Polyclonal Antibody, Unconjugated (bs-0932R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-WDR26 Polyclonal Antibody, Unconjugated (bs-0932R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF-7 cells were stained with WDR26 Polyclonal Antibody, Unconjugated(bs-0932R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
RSC96 cells probed with WDR26 Polyclonal Antibody, unconjugated (bs-0932R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Catalog #1: bs-0932R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human rectal tissue labeled with Anti-Adiponectin Receptor 1 Polyclonal Antibody (bs-0610R), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining
Mouse bone lysates probed with Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated (bs-0610R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Muscle tissue lysates; Lane 2: Mouse Breast tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Rat Muscle tissue lysates; Lane 5: Rat Heart tissue lysates probed with Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated (bs-0610R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0610R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse brain lysates and Human A549 cells probed with PACAP receptor 1 Polyclonal Antibody, unconjugated (bs-0198R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with PACAP receptor Polyclonal Antibody, Unconjugated (bs-0198R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0198R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Nociceptin Polyclonal Antibody, Unconjugated(bs-0075R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 rat liver lysates probed (bs-0075R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 19kDa.

Catalog #1: bs-0075R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-0432R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human rectal tissue labeled with Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Heart lysates; Lane 2: Mouse Skeletal muscle lysates; Lane 3: Rat Heart lysates; Lane 4: Rat Skeletal muscle lysates; Lane 5: Human 293T cell lysates probed with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Adiponectin receptor 2) Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0611r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated(bsm-52289R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Caspase-3 Monoclonal Antibody, Unconjugated(bsm-52289R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PSMA8 Polyclonal Antibody, Unconjugated (bs-19461R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PSMA8 Polyclonal Antibody, Unconjugated (bs-19461R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

Catalog #1: bsm-52289r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Anti-ADFP Polyclonal Antibody, Unconjugated (bs-1164R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse liver lysates probed with ADFP Polyclonal Antibody, Unconjugated (bs-1164R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1164R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 (9E7) Monoclonal Antibody, Unconjugated(bsm-52009R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 (9E7) Monoclonal Antibody, Unconjugated(bsm-52009R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 (9E7) Monoclonal Antibody, Unconjugated(bsm-52009R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM17 (9E7) Monoclonal Antibody, Unconjugated(bsm-52009R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52009r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Ribonuclease Inhibitor Polyclonal Antibody, Unconjugated (bs-7518R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Ribonuclease Inhibitor Polyclonal Antibody, Unconjugated (bs-7518R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Antigen: bs7518P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in Microplate Reader by 450nm\\n
Lane 1: mouse intestine lysates Lane 2: mouse brain lysates probed with Anti Ribonuclease Inhibitor Polyclonal Antibody, Unconjugated (bs-7518R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 50kD. Observed band size: 50kD.

Catalog #1: bs-7518R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-EDG1\/CD363\/S1P1 Polyclonal Antibody, Unconjugated (bs-7112R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Rat heart lysates probed with S1P1 Polyclonal Antibody, unconjugated (bs-7112R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: HUVEC Cells; Probed with S1P1 Polyclonal Antibody (bs-7112R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Cerebellum tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Cerebellum tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with EDG1 Polyclonal Antibody, Unconjugated (bs-7112R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7112R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7022R

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RERE Polyclonal Antibody, Unconjugated (bs-7077R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RERE Polyclonal Antibody, Unconjugated (bs-7077R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-7077R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse pancreas labeled with Anti-TMEM49 Polyclonal Antibody, Unconjugated (bs-6740R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Mouse kidney lysates probed with TMEM49 Polyclonal Antibody, Unconjugated (bs-6740R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Stomach tissue lysates; Lane 2: Mouse Cerebrum tissue lysates probed with TMEM49 Polyclonal Antibody, Unconjugated (bs-6740R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6740R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RAB7 Polyclonal Antibody(bs-6703R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RAB7 Polyclonal Antibody, Unconjugated (bs-6703R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human HL-60 cell lysates probed with RAB7 Polyclonal Antibody, Unconjugated (bs-6703R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6703R

Applications: WB, ELISA, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-6711R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: RAW264.7 (Mouse) Cell Lysate probed with Alpha-synuclein(Ser129) Antibody, Unconjugated (bs-5628R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebellum lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Cerebellum lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human U251 cell lysates; Lane 7: Human 293T cell lysates; Lane 8: Human Hela cell lysates probed with Alpha synuclein Polyclonal Antibody, Unconjugated (bs-5628R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human SY5Y cell lysates; Lane 2: Human U87MG lysates; Lane 3: Human U251 cell lysates; Lane 4: Human HUVEC cell lysates; Lane 5: Human HELA cell lysates; Lane 6: Human 293T cell lysates probed with phospho-Alpha synuclein (Ser129) Antibody, Unconjugated (bs-5628R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5628R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DDX58 (Thr170) Polyclonal Antibody, Unconjugated (bs-5300R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human MOLT4 cell lysates; Lane 4: Human MCF-7 cell lysates probed with phospho-DDX58 (Thr170) Polyclonal Antibody, Unconjugated (bs-5300R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5300R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti Nur77 Polyclonal Antibody (bs-3513R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Sample: <br>\r\nLane 1: Human Jurkat cell lysates<br>\r\nLane 2: Human HeLa cell lysates<br>\r\nPrimary: Anti-Nur77 (bs-3513R) at 1\/1000 dilution<br>\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution<br>\r\nPredicted band size: 66 kDa<br>\r\nObserved band size: 73 kDa<br>\r\nLot: AI11268723\r\n

Catalog #1: bs-3513R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-LC3B\/MAP LC3\u03b2\/MAP1A Polyclonal Antibody, Unconjugated (bs-2912R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Recombinant human LC3B protein, N-His(bs-42305P) probed with LC3B Polyclonal Antibody, Unconjugated (bs-2912R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-2912r

Applications: IHC-P, IHC-F, IF

Reactivity: Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-2601R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2527R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-2527R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-2485R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Catalog #1: bs-2284R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with SLC26A6 Polyclonal Antibody, Unconjugated \\tbs-20817R at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SLC26A6 Polyclonal Antibody, Unconjugated (bs-20817R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SLC26A6 Polyclonal Antibody, Unconjugated (bs-20817R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-20817R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded pig liver labeled with Anti-CK18 Polyclonal Antibody, Cy3 Conjugated (bs-2043R-Cy3) at 1:200 for 60 minutes at 37C.
PC12 cells probed with Rabbit Anti-CK18 Polyclonal Antibody (bs-2043R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Paraformaldehyde-fixed, paraffin embedded human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CK18 Polyclonal Antibody, Unconjugated (bs-2043R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Rat Large intestine lysates probed with Cytokeratin 18 Polyclonal Antibody, Unconjugated (bs-2043R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-2043R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-PEG3 Polyclonal Antibody, Unconjugated(bs-1870R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
SH-SY5Y cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with PEG3 Polyclonal Antibody(bs-1870R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PEG3) Polyclonal Antibody, Unconjugated (bs-1870R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PEG3) Polyclonal Antibody, Unconjugated (bs-1870R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1870R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CLEC9A Polyclonal Antibody, Unconjugated (bs-13621R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CLEC9A Polyclonal Antibody, Unconjugated (bs-13621R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.

Catalog #1: bs-13621R

Applications: WB, ELISA, IHC-P, IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: MCF-7 cell lysates probed with FAK Polyclonal Antibody, Unconjugated (bs-1340R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Rat Cerebrum lysates; Lane 4: Human 293T cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human HUVEC cell lysates; Lane 7: Human A549 cell lysates probed with FAK Polyclonal Antibody, Unconjugated (bs-1340R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (FAK) polyclonal Antibody, Unconjugated (bs-1340R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (bs-1340R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1340R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12998r-TR

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-GPA33 Polyclonal Antibody, Unconjugated (bs-1226R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-GPA33 Polyclonal Antibody, Unconjugated (bs-1226R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-1226R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TAZ Polyclonal Antibody, Unconjugated (bs-12367R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: mouse lung lysates probed with \\tTAZ Polyclonal Antibody, Unconjugated (bs-12367R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TAZ Polyclonal Antibody, Unconjugated (bs-12367R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
A549 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nTAZ Polyclonal Antibody(bs-12367R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-12367R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11745R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10341R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10407R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Independently Validated Antibody, image provided by Science Direct, badge number 028752. Western blot analysis of mouse brain and liver extracts using NK2 Homeobox 1 (bs-0826R NKX2-1) antibody, 1:500 dilution. NKX2-1 is present in the positive control sample (brain) and absent from the negative control sample (liver). The predicted and observed position of NKX2-1 is at around 38kDa.
PFA fixed A549 cells with Anti-TTF1 Polyclonal Antibody (bs-0826R) at 1:200 dilution, followed by conjugation to the secondary, Goat Anti-Rabbit IgG A488 1:100 for 30min. This data was generously submitted by an end user as part of our Bioss Discovery program.
Human A549 probed with TTF1 Polyclonal Antibody, Unconjugated (bs-0826R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Lane 1: Mouse Thyroid gland Lysates; Lane 2: Rat Thyroid gland Lysates. Probed with TTF1\u00a0 polyclonal Antibody, unconjugated (bs-0826R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0826R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0699R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-0947R-FITC

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-phospho-FSCN1(Ser39) Polyclonal Antibody, Unconjugated (bs-0772R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
U87MG Cell lysates probed with FSCN1(Ser39) Polyclonal Antibody, Unconjugated (bs-0772R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibodyincubation at 1:20000 for 60 min at 37˚C.\r\n
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FSCN1(Ser39) Polyclonal Antibody(bs-0772R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-FSCN1 (Ser39)) polyclonal Antibody, Unconjugated (bs-0772R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0772R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse splenocytes probed with Rabbit Anti-JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with JAK2 Polyclonal Antibody(bs-0908R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human K562 cell lysates; Lane 2: Mouse BV-2 cell lysates probed with JAK2 Polyclonal Antibody, Unconjugated (bs-0908R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0908R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Jurkat lysates probed with PKA alpha + beta Polyclonal Antibody, Unconjugated (bs-0520R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PKA alpha + beta) polyclonal Antibody, Unconjugated (bs-0520R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Heart tissue lysates probed with PKA alpha + beta Polyclonal Antibody, Unconjugated (bs-0520R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0520R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with progesterone receptor Antibody(bs-0111R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF-7 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Progesterone Receptor Antibody(bs-0111R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human MCF-7 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human Hela cell lysates probed with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-0111R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0111R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

\"Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-HSP-70 Polyclonal Antibody (bs-0126R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n\"
Generously provided by Merlin Levine from National Institutes of Health as part of the Bioss Discovery Program. Mouse brain labeled with Anti-HSP70 Polyclonal Antibody, Unconjugated (bs-0126R) at 1:100 overnight. The secondary antibody was AlexaFluor 555 goat anti mouse IgG1 used at 1:250 for one hour.
Mouse bladder lysates probed with HSP70 Polyclonal Antibody, Unconjugated (bs-0126R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HSP70) polyclonal Antibody, Unconjugated (bs-0126R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0126R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated