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Western Blot Antibodies



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Catalog #1: bs-19143R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded Mouse Placenta tissue labeled with CD274 Polyclonal Antibody, Unconjugated (bs-10159R) at 1:200 followed by incubation with a secondary antibody, DAB and counter-staining
Mouse Spleen Cells were fixed with 70% methanol (Overnight at 4\u2103). The cells were then incubated in 1 X PBS\/2%BSA\/10% goat serum to block non-specific protein-protein interactions followed by incubation with CD274 Polyclonal Antibody, PE-Conjugated (bs-10159R-PE) at 1:100 for 30 minutes at room temp. The graph compares the primary antibody (green) to unstained cells (dark blue), and isotype control (orange; bs-0295P-PE).
Lane 1: Du145 cell lysates; Lane 2: Lovo cell lysates; Lane 3: Mouse Heart lysates; Lane 4: Mouse Muscle lysates ; Lane 5: Rat Heart lysates probed with CD274 Polyclonal Antibody, Unconjugated (bs-10159R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD274 Polyclonal Antibody(bs-10159R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-10159R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8033R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-APEX2 Polyclonal Antibody, Unconjugated (bs-6587R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human A549 cell lysates; Lane 4: Human U87MG cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human MCF-7 cell lysates; Lane 7: Human HeLa cell lysates; Lane 8: Human LOVO cell lysates probed with APEX2 Polyclonal Antibody, Unconjugated (bs-6587R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6587R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon labeled with Anti OLFM4 Polyclonal Antibody, Unconjugated (bs-6558R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse bone lysates probed with Rabbit Anti- Polyclonal Antibody, Unconjugated (bs-R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

Catalog #1: bs-6558R

Applications: WB, ELISA, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain glioma tissue labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 \u00b0C followed by conjugation to secondary antibody.
Images provided the Independent Validation Program (badge number 029629)Formalin-fixed and paraffin embedded human brain tissue with Parkinson's morphology labeled with Rabbit Anti-WNT2 Polyclonal Antibody (bs-6133R) at 1:250 overnight at 4 \u00b0C followed by conjugation to secondary antibody.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with WNT2 Polyclonal Antibody, Unconjugated (bs-6133R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human HeLa cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human Jurkat cell lysates probed with WNT2 Polyclonal Antibody, Unconjugated (bs-6133R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6133R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPA2(Thr21) Polyclonal Antibody, Unconjugated (bs-5693R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPA2(Thr21) Polyclonal Antibody, Unconjugated (bs-5693R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPA2(Thr21) Polyclonal Antibody, Unconjugated (bs-5693R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5693R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat liver lysates Lane 2: rat brain lysates probed with Anti CK12\/Cytokeratin 12 Polyclonal Antibody, Unconjugated (bs-4625R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 52kD. Observed band size: 52kD.
Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-CK12 Polyclonal Antibody, Unconjugated (bs-4625R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-4625R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-TUBB3 Polyclonal Antibody, Unconjugated (bs-4512R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 rat kidney lysates probed with Anti TUBB3 Polyclonal Antibody, Unconjugated (bs-4512R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 50kD. Observed band size:50kD.\\n
Tissue\/cell: BV-2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with beta III Tubulin Polyclonal Antibody, Unconjugated (bs-4512R) 1:200, 90 minutes at 37\u00b0C; followed by a conjugated secondary antibody (bs-0295G-FITC) at 37\u00b0C for 90 minutes, DAPI (5ug\/ml) was used to stain the cell nuclei.
SH-SY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta 3 Tubulin Polyclonal Antibody(bs-4512R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-4512R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human 293T Cell lysate; Probed with Tie2(Tyr992) Polyclonal Antibody, Unconjugated (bs-3449R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin-embedded Human Liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tie2 (Tyr992) Polyclonal Antibody, Unconjugated (bs-3449R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Phospho-Tie2 (Tyr992)) Polyclonal Antibody, Unconjugated (bs-3449R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Phospho-Tie2 (Tyr992)) Polyclonal Antibody, Unconjugated (bs-3449R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3449R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2706R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Parkin protein\/PARK2 Polyclonal Antibody, Unconjugated (bs-1865R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat testis labeled with Anti-Parkin protein\/PARK2 Polyclonal Antibody, Unconjugated (bs-1865R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Parkin\/PARK2) Polyclonal Antibody, Unconjugated (bs-1865R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1865R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Anti-LIN9 Polyclonal Antibody, Unconjugated (bs-18283R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\\n
Mouse bone lysates probed with Anti-LIN9 Polyclonal Antibody, Unconjugated (bs-18283R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:10000 for 90 min at 37˚C.\\n
25 ug total protein per lane of various lysates (see on figure) probed with LIN9 polyclonal antibody, unconjugated (bs-18283R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-18283r-TR

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-1693R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical tissue labeled with Anti-XBP-1 Polyclonal Antibody, Unconjugated (bs-1668R) followed by conjugation to the secondary antibody and DAB staining
L1 mouse embryo lysate L2 mouse liver lysates probed with Anti XBP1 Polyclonal Antibody, Unconjugated (bs-1668R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 29kD. Observed band size: 29kD
Lane 1: Mouse Large intestine lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Testis lysates; Lane 4: HepG2 cell lysates probed with XBP1 Polyclonal Antibody, Unconjugated (bs-1668R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (XBP1) polyclonal Antibody, Unconjugated (bs-1668R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-1668R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney cancer labeled with Anti-HIF-2\u03b1 Polyclonal Antibody, Unconjugated (bs-1447R) followed by conjugation to the secondary antibody and DAB staining
Rat lung lysates probed with Rabbit Anti-HIF2 alpha Polyclonal Antibody, Unconjugated (bs-1447R) at 1:200 and Mouse Anti-GAPDH (bsm-0978M) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
HepG2 cells(black) were fixed with 70% ice-cold methanol overnight at 4\u2103,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HIF2 alpha Polyclonal Antibody(bs-1447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1447R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human brain with labeled Anti-TIE1 Polyclonal Antibody, Unconjugated (bs-1334R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1:A549 cell lysates; Lane 2: HepG2 cell lysates probed with TIE1 Polyclonal Antibody, Unconjugated (bs-1334R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1334R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti-HOXA13 Polyclonal Antibody, Unconjugated (bs-12244R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Rat Liver tissue lysates; Lane 4: Rat Uterus tissue lysates ; Lane 5: Human 293T cell lysates; Lane 6: Human Hela cell lysates; Lane 7: Human U2os cell lysates probed with HOXA13 Polyclonal Antibody, Unconjugated (bs-12244R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n

Catalog #1: bs-12244R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12362R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-11240R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-11451R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human laryngeal carcinoma labeled with Rabbit Anti-CD276\/B7H3 Polyclonal Antibody, Unconjugated (bs-11019R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1:JAR cell lysates;Lane 2: MCF-7 cell lysates probed with CD276 Polyclonal Antibody, Unconjugated (bs-11019R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-11019R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rabbit heart labeled with Anti CTn1\/Troponin I\/TNNC1 Polyclonal Antibody,Unconjugated (bs-0799R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse heart tissue with Anti-CTn1\/Troponin I\/TNNC1 Polyclonal Antibody, Unconjugated (bs-0799R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cardiac Troponin I) Polyclonal Antibody, Unconjugated (bs-0799R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Heart tissue lysates; Lane 2: Rat Heart tissue lysates probed with TNNI3 Polyclonal Antibody, Unconjugated (bs-0799R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Catalog #1: bs-0799R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10042R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0136R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Annexin A13 Polyclonal Antibody, Unconjugated (bs-0055R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Artemin) Polyclonal Antibody, Unconjugated (bs-0055R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0055R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon carcinoma labeled with Anti-Slc22A17 Y Polyclonal Antibody, Unconjugated (bs-0444R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human U-2OS cell lysates; Lane 3: Human U-87MG cell lysates; Lane 4: Human A549 cell lysates; Lane 5: Human Siha cell lysates probed with Slc22A17 Polyclonal Antibody, Unconjugated (bs-0444R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0444R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-EphB2 R Polyclonal Antibody, Unconjugated (bs-0247R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-EphB2 R Polyclonal Antibody, Unconjugated (bs-0247R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Mouse testis lysates probed with Rabbit Anti-EphB2 R Polyclonal Antibody, Unconjugated (bs-0247R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody for 90 min at 37˚C.
Lane 1: Mouse Testis lysates; Lane 2: Rat Testis lysates; Lane 3: Human HUVEC cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human U-2OS cell lysates; Lane 7: Human MDA-MB-231 cell lysates; Lane 8: Human K562 cell lysates probed with EphB2 R Polyclonal Antibody, Unconjugated (bs-0247R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0247R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HepG2 lysates; Lane 2: Hcclm3 lysates probed with BTG2 Polyclonal Antibody, Unconjugated (bs-0031R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human K562 cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HepG2 cell lysates probed with BTG2 Polyclonal Antibody, Unconjugated (bs-0031R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0031R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0295G-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Cyanine 5

IF(ICC) staining with CD14 (31G4) Monoclonal Antibody (bsm-52556R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CD14 (31G4) Monoclonal Antibody (bsm-52556R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CD14 (31G4) Monoclonal Antibody (bsm-52556R) at 1:100 in LO2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD14 (31G4) Monoclonal Antibody, Unconjugated (bsm-52556R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52556r

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

IF(ICC) staining with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54290r-100ul

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11598R

Applications: WB, ELISA, IHC-P, IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon cancer labeled with Rabbit Anti-OGG1 Polyclonal Antibody, Unconjugated(bs-3687R) 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with OGG1 Polyclonal Antibody, Unconjugated (bs-3687R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with OGG1 Polyclonal Antibody, Unconjugated (bs-3687R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with OGG1 Polyclonal Antibody, Unconjugated (bs-3687R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3687R

Applications: WB, ELISA, FCM, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse spleen lysates probed with Activated Notch1 Polyclonal Antibody, unconjugated (bs-20252R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded rat spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Activated Notch1 Polyclonal Antibody, Unconjugated bs-20252R at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Activated Notch1 Polyclonal Antibody, Unconjugated (bs-20252R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Activated Notch1 Polyclonal Antibody, Unconjugated (bs-20252R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-20252R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Human breast milk Lysate Lane 1: 2 uL; Lane 2: 6 uL; Lane 3: 8uL probed with Alpha casein Polyclonal Antibody, Unconjugated (bs-8245R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Milk lysates probed with Alpha casein Polyclonal Antibody, Unconjugated (bs-8245R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-8245R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LATS1 (Thr1079) Polyclonal Antibody (bs-7913R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Hela cell lysates probed with LATS1+LATS2 (Thr1079 +Thr1041) Polyclonal Antibody, Unconjugated (bs-7913R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse Cerebrum lysates probed with LATS1+LATS2 (Thr1079 +Thr1041) Polyclonal Antibody, Unconjugated (bs-7913R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7913r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rabbit

Conjugate: Unconjugated

Lane 1: Mouse Brain; Lane 2: Rat Brain; Probed with Flotillin 1 Polyclonal Antibody, Unconjugated (bs-7798R) at 1:500 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37\u00b0C.
Lane 1: Mouse Heart tissue lysates; Lane 2: Rat Heart tissue lysates probed with Flotillin 1 Polyclonal Antibody, Unconjugated (bs-7798R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7798R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-PAI1\/PLANH1\/Serpine 1 Polyclonal Antibody, Unconjugated(bs-6562R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.DAPI was used to stain the cell nuclei
Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PAI1) Polyclonal Antibody, Unconjugated (bs-6562R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PAI1) Polyclonal Antibody, Unconjugated (bs-6562R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-6562R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CKMT\/Creatine kinase MT Polyclonal Antibody, Unconjugated (bs-6522R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti CKMT\/Creatine kinase MT Polyclonal Antibody, Unconjugated (bs-6522R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti CKMT\/Creatine kinase MT Polyclonal Antibody, Unconjugated (bs-6522R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse small intestine lysates probed with CKMT\/Mitochondrial Creatine Kinase Polyclonal Antibody, Unconjugated (bs-6522R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6522R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Anti ANGPTL3\/ANG5 Polyclonal Antibody, Unconjugated (bs-5918R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HepG2 lysates probed with ANGPTL3 Polyclonal Antibody, Unconjugated (bs-5918R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human HL-60 cell Lysates; Lane 2: Human HepG2 cell Lysates; Lane 3: Human 293T cell Lysates; Lane 4: Human Raji cell Lysates; Lane 5: Human MOLT4 cell Lysates. Probed with ANGPTL3 polyclonal Antibody, unconjugated (bs-5918R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5918R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-5795R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-5599R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-5589R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-PI 3 Kinase p110 delta (Tyr524) Polyclonal Antibody (bs-5587R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Rat Thymus lysates; Lane 2: Human HT-1080 cell lysates. Probed with phospho-PI 3 Kinase p110 delta (Tyr524) polyclonal Antibody, unconjugated (bs-5587R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5587R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse small intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Layilin Polyclonal Antibody, Unconjugated (bs-4629R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Layilin) Polyclonal Antibody, Unconjugated (bs-4629R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (Goat Anti-rabbit IgG\/Bio) for 20minutes at 37\u00b0C, followed by a conjugated streptavidin for 40 minutes and DAPI staining of the nuclei.
Paraformaldehyde-fixed, paraffin embedded mouse intestines; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Layilin) Polyclonal Antibody, Unconjugated (bs-4629R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary (Goat Anti-rabbit IgG\/Bio) for 20minutes at 37\u00b0C, followed by a conjugated streptavidin for 40 minutes and DAPI staining of the nuclei.

Catalog #1: bs-4629R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti SOAT2\/ACAT2 Polyclonal Antibody, Unconjugated (bs-5020R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human colon labeled with Anti-SOAT2\/ACAT2 Polyclonal Antibody, Unconjugated(bs-5020R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
L1 rat intestine lysates L2 rat brain lysates probed with Anti SOAT2\/ACAT2 Polyclonal Antibody, Unconjugated (bs-5020R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 57kD. Observed band size:57kD.
Lane 1: Mouse Liver tissue lysates; Lane 2: Rat Liver tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human K562 cell lysates probed with SOAT2 Polyclonal Antibody, Unconjugated (bs-5020R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5020R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-LONP1 Polyclonal Antibody, Unconjugated (bs-4245R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti- LONP1 Polyclonal Antibody, Unconjugated (bs-4245R) at 1:200 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded rat colon labeled with Anti- LONP1 Polyclonal Antibody, Unconjugated (bs-4245R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C
L1 mouse kidney lysates L2 mouse intestine lysates probed with Anti LONP1 Polyclonal Antibody, Unconjugated (bs-4245R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 98kD. Observed band size: 98kD

Catalog #1: bs-4245R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated