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Catalog #1: bs-7106R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Mouse spleen lysates probed with CD81 Polyclonal Antibody, Unconjugated (bs-6934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Jurkat cell lysates probed with CD81 Polyclonal Antibody, Unconjugated (bs-6934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1:U937 cell lysates probed with CD81 Polyclonal Antibody, Unconjugated (bs-6934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse Lung lysates; Lane 4: Mouse Thymus lysates; Lane 5: Mouse RAW264.7 cell lysates; Lane 6: Human THP-1 cell lysates; Lane 7: Human Raji cell lysates probed with CD81 Polyclonal Antibody, Unconjugated (bs-6934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6934R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti Phospho-TAK1(Thr184\/187) Polyclonal Antibody, Unconjugated (bs-3439R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Phospho-TAK1(Thr184\/187) Polyclonal Antibody, Unconjugated(bs-3439R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-3439R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Image provided by Independent Validation Program, badge 29801.\\n\\nLane 1: HeLa G2\/M Phase cell lysate. Lane 2: C6\/36 cell lysates probed with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Antibody, Unconjugated (bs-3332R) at 1:200 for 10 hours at 4˚C. Followed by conjugation to secondary antibody at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated (bs-3332R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Catalog #1: bs-3332R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2389R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-phospho-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human stomach cancer tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Kindey lysates probed with STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1658R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Mouse kidney lysates probed with Rabbit Anti-ETL\/ELTD1 Polyclonal Antibody, Unconjugated (bs-13111R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue).
Paraformaldehyde-fixed, paraffin embedded rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with ETL\/ELTD1 Polyclonal Antibody, Unconjugated (bs-13111R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tETL\/ELTD1 Polyclonal Antibody, Unconjugated (bs-13111R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining.

Catalog #1: bs-13111R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Rabbit Anti CD138\/Syndecan-1 Polyclonal Antibody, Unconjugated (bs-1309R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 human colon carcinoma lysates probed with Anti- CD138\/Syndecan-1 Polyclonal Antibody, Unconjugated (bs-1309R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 34kD.Observed band 34D, 92kD
Paraformaldehyde-fixed, paraffin embedded rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \r\nSyndecan 1 Polyclonal Antibody, Unconjugated (bs-1309R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and for 20 minutes and DAB staining.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Human U251 cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human HeLa cell lysates probed with Syndecan-1 Polyclonal Antibody, Unconjugated (bs-1309R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1309R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: Unconjugated

Lane 1: Lovo cell lysates; Lane 2: MCF-7 cell lysates probed with Neurotensin Receptor 1 Polyclonal Antibody, Unconjugated (bs-12002R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: NTSR1 KO Human 293T cell lysates; Lane 2: NTSR1 KO Human 293T cell lysates; Lane 3: Human 293T cell lysates probed with Neurotensin Receptor 1 Polyclonal Antibody, Unconjugated (bs-12002R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-12002R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti Arginase II Polyclonal Antibody, Unconjugated (bs-11397R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Anti-Arginase II Polyclonal Antibody, Unconjugated (bs-11397R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
DU145 Cell lysates; probed with Arginase II Polyclonal Antibody, unconjugated (bs-11397R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Arginase II) polyclonal Antibody, Unconjugated (bs-11397R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-11397R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-EAAT1\/GLAST Polyclonal Antibody (bs-1003R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti EAAT1\/Glast Polyclonal Antibody, Unconjugated (bs-1003R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 56kD. Observed band size: 56kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (EAAT1) Polyclonal Antibody, Unconjugated (bs-1003R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (EAAT1) Polyclonal Antibody, Unconjugated (bs-1003R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1003R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Images provided by the Independent Validation Program (badge number 29749). Lane 1: A549 cell extract Lane 2: c6\/36 mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
HeLa cells probed with PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PALB2) polyclonal Antibody, Unconjugated (bs-0588R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0588R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Pig

Conjugate: Unconjugated

Rat cerebrum lysates probed with PACAP-27\/38 Polyclonal Antibody, Unconjugated (bs-0190R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat cerebrum lysates probed with PACAP-27\/38 Polyclonal Antibody, Unconjugated (bs-0190R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0190R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-MMP-7 Polyclonal Antibody, Unconjugated(bs-0423R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti-MMP-7 Polyclonal Antibody, Unconjugated (bs-0423R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse Uterus lysates probed with MMP-7 Polyclonal Antibody, unconjugated (bs-0423R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP7 Polyclonal Antibody, Unconjugated (bs-0423R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

Catalog #1: bs-0423r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Human HT-29 lysates probed with Rabbit Anti-Glut2 Polyclonal Antibody, Unconjugated (bs-0351R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Hep G2 cells probed with GLUT2 Polyclonal Antibody, unconjugated (bs-0351R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Lane 1: Mouse embyo lysates probed with GLUT2 Polyclonal Antibody, Unconjugated (bs-0351R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0351R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-GAS 6 Polyclonal Antibody, Unconjugated (bs-7549R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse liver lysates Lane 2: Mouse kidney lysates probed with Rabbit Anti-GAS 6 Polyclonal Antibody, Unconjugated (bs-7549R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-7549R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Antigen: bs-4572P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti VEGF-A Polyclonal Antibody, Unconjugated (bs-4572R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with VEGF-A Polyclonal Antibody(bs-4572R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-4572R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, GuineaPig

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse hindfoot tumor labeled with Anti-\u00b5-Opioid receptor Polyclonal Antibody, Unconjugated (bs-3623R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse skin labeled with Anti-\u00b5-Opioid receptor Polyclonal Antibody, Unconjugated (bs-3623R) followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-3623R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti Phospho-PKR (Thr446 + Thr451) Polyclonal Antibody (bs-3337R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PKR(Thr446+Thr451) Polyclonal Antibody, Unconjugated (bs-3337R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining\\t\\t\\t\\t\\t\\t\\t\\t\\t\\t\\t\\t\\t\\t\\t
Mouse spleen and liver lysates probed with PKR(Thr446+Thr451) Polyclonal Antibody, unconjugated (bs-3337R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n
Lane 1: Human HeLa cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human 293T cell lysates; Lane 4: Human A431 cell lysates probed with Phospho-PKR (Thr446 + Thr451) Polyclonal Antibody, Unconjugated (bs-3337R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3337R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated(bs-1992R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (bs-1992R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE)and DAPI staining
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mTOR\/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mTOR\/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1992R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-11 Polyclonal Antibody, Unconjugated (bs-1827R) at 1:400 overnight at 4\u00b0C, DAB staining.
Mouse cerebellum lysates probed with IL-11 Polyclonal Antibody, Unconjugated (bs-1827R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates probed with IL-11 Polyclonal Antibody, Unconjugated (bs-1827R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1827R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ADRB3\/beta 3 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-1063R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-beta 3 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-1063R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-1063R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti-CD36\/PAS-4 Polyclonal Antibody, Unconjugated (bs-1100R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse heart lysates probed with Rabbit Anti-CD36 Polyclonal Antibody, Unconjugated (bs-1100R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Rat Placenta tissue lysates; Lane 4: Human HUVEC cell lysates; Lane 5: Human HL-60 cell lysates probed with CD36 Polyclonal Antibody, Unconjugated (bs-1100R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD36 Polyclonal Antibody, Unconjugated(bs-1100R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bs-1100r-TR

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-PEGF Polyclonal Antibody, Unconjugated (bs-0731R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat eye, ciliary body labeled Anti-PEGF Polyclonal Antibody, Unconjugated (bs-0731R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-0731R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-SCF Polyclonal Antibody (bs-0545R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Mouse spleen cells were fixed with 4% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were then stained with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:33 for 30 min at room temperature. A Goat anti-rabbit IgG-AF647 secondary antibody was used at 1ug for 40 min at room temperature. Acquisition of 10,000 events was performed. Primary antibody staining (green) is compared to compared to unstained cells (purple), secondary only (light blue), and isotype control (orange).
Sp2\/0 (Mouse myeloma cell) lysates probed with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
U87MG cells were fixed with 4% PFA (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were then stained with SCF Polyclonal Antibody, Unconjugated (bs-0545R) at 1:100 for 30 min at room temperature. A Goat anti-rabbit IgG-PE (bs-0295G-PE) secondary antibody was used at 1ug for 40 min at room temperature. Acquisition of 20,000 events was performed. Primary antibody staining (green) is compared to compared to unstained cells (purple), secondary only (light blue), and isotype control (orange).

Catalog #1: bs-0545R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat transplant lymphoma labeled with Anti-Caspase-1 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF-7 Cells lysates probed with Caspase-1 P10 Polyclonal Antibody, unconjugated (bs-0169R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Caspase-1 P10 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: U-87MG Cells; 30ug loaded into the lane; Probed with Caspase-1 P10 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-0169R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0295p-cy5-100ul

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Dopamine D2 Receptor Polyclonal Antibody, Unconjugated (bs-20729R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Dopamine D2 Receptor Polyclonal Antibody, Unconjugated (bs-20729R) at 1:400 overnight at 4\u00b0C, DAB staining.
Rat Cerebrum lysates probed with Dopamine D2 Receptor Polyclonal Antibody, Unconjugated (bs-20729R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-20729R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6663R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, FITC Conjugated (bsm-0917M-FITC) at 1:200 for 40 minutes at 37\u00b0C followed by labeling Rabbit Anti-Tubulin Beta, Cy3 conjugated(bs-4511R-Cy3) 1:200, 40 minutes at 37\u00b0C
Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 50kD. Observed band size: 50kD.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1:Mouse heart lysates; Lane 2: Human MCF7 cell lysates probed with Rabbit Anti-Tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-4511R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4121R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-ID1 Polyclonal Antibody, Unconjugated (bs-3529R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-ID1 Polyclonal Antibody, Unconjugated (bs-3529R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-ID1 Polyclonal Antibody, Unconjugated (bs-3529R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-3529R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse kidney lysates Lane 2: mouse lung lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated (bs-2602R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 39kD. Observed band size: 39kD.
Lane 1: HepG2 cell lysates; Lane 2: Mouse Lung lysates; Lane 3: Rat Lung lysates probed with IL10RB Polyclonal Antibody, Unconjugated (bs-2602R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2602R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse liver lysates probed with Rabbit Anti-AQP9 Polyclonal Antibody, Unconjugated (bs-2060R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-AQP9 Polyclonal Antibody, Unconjugated (bs-2060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: Mouse intestine lysates probed with Rabbit Anti-AQP9 Polyclonal Antibody, Unconjugated (bs-2060R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-2060R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical tissue labeled with Anti-CNR1 Polyclonal Antibody, Unconjugated (bs-1683R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
SH-SY5Y cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CNR1 Polyclonal Antibody(bs-1683R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-1683R

Applications: IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-PCX\/PODXL Polyclonal Antibody, Unconjugated (bs-1345R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-PCX\/PODXL Polyclonal Antibody, Unconjugated (bs-1345R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1345r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-13130R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat lung labeled with Anti-TRAIL Polyclonal Antibody, Unconjugated (bs-1214R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse spleen tissue labeled with Anti-TRAIL Polyclonal Antibody, Unconjugated (bs-1214R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse lung lysates; Lane 2: U251 cell lysates probed with Anti\u2013TRAIL Polyclonal Antibody, Unconjugated (bs-1214R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-1214R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with\\tSPOCK2\/Testican 2 Polyclonal Antibody, Unconjugated ( \\tbs-11966R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Human Hela cells probed with SPOCK2\/Testican 2 Polyclonal Antibody, unconjugated (bs-11966R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SPOCK2\/Testican 2 Polyclonal Antibody, Unconjugated (bs-11966R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-11966R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Mouse brain cells probed with Rabbit Anti-PTP zeta Polyclonal Antibody, FITC Conjugated (bs-11327R-FITC) (green) at 1:50 for 40 minutes compared to control cells (red).
Image obtained from One World Lab validations program. A431 cells were stained with PTP zeta Polyclonal Antibody, Unconjugated (bs-11327R) at 1:50 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #1: bs-11327R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Desmin Polyclonal Antibody, Unconjugated (bs-1026R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse heart tissue labeled with Anti-Desmin Polyclonal Antibody, Unconjugated (bs-1026R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 rat intestine lysates probed with Anti Desmin\/DES Polyclonal Antibody, Unconjugated (bs-1026R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 52kD. Observed band size:52kD.\\n

Catalog #1: bs-1026R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Cyclooxygenase 2\/COX2 Polyclonal Antibody, Unconjugated (bs-0732R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-0732P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Human A549 lysates probed with Rabbit Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated (bs-0732R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Mouse brain lysates probed with Rabbit Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated (bs-0732R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.

Catalog #1: bs-0732R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated (bs-0664R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Macrophages probed with Rabbit Anti-HMGB1 Polyclonal Antibody, Unconjugated (bs-0664R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Human HepG2 cell lysates probed with Rabbit Anti-HMGB1 Polyclonal Antibody, PE-Cy5 conjugated (bs-0664R-PE-Cy5) (green) at 1:20 for 30 minutes followed compared to unstained cells (blue) and PE-Cy5 isotype control bs-0295P-PE-Cy5(orange).
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HMGB1 Polyclonal Antibody(bs-0664R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0664R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-PMP22 Polyclonal Antibody, Unconjugated (bs-0235R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat spinal cord labeled with Anti-PMP22 Polyclonal Antibody, Unconjugated (bs-0235R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

Catalog #1: bs-0235R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0406R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded rat brain labeled with CEMIP Polyclonal Antibody, Unconjugated (bs-8059R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CEMIP Polyclonal Antibody, Unconjugated (bs-8059R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CEMIP Polyclonal Antibody, Unconjugated (bs-8059R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CEMIP Polyclonal Antibody, Unconjugated (bs-8059R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bs-8059R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Human 293T cells probed with CD32B(Tyr292) Polyclonal Antibody, unconjugated (bs-6031R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD32B(Tyr292) Polyclonal Antibody, Unconjugated (bs-6028R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-6031R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat testis labeled with Anti-SOX9\/SRA1 Polyclonal Antibody, Unconjugated (bs-4177R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
SW480 and Ht29 lysates probed with SOX9 Polyclonal Antibody, unconjugated (bs-4177R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
U-251 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SOX9 Polyclonal Antibody(bs-4177R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
293T cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SOX9 Antibody(bs-4177R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-4177R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated