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Western Blot Antibodies



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Catalog #1: bsm-0978m-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: PE-Cy7

Catalog #1: bsm-0978m-bf750-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: AbBy Fluor-750

Catalog #1: bsm-0978m-percp-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: PerCP

Catalog #1: bsm-0978m-cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: Cy5.5

Catalog #1: bsm-0978m-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: Cy5

Catalog #1: bsm-0978m-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: PE-Cy5

Catalog #1: bsm-0978m-percp-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: PerCP-Cy7

Catalog #1: bsm-0978m-bf594-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: AbBy Fluor-594

Catalog #1: bsm-0978m-bf488-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: AbBy Fluor-488

Catalog #1: bsm-0978m-bf647-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: AbBy Fluor-647

Catalog #1: bsm-0978m-pe-cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: PE-Cy5.5

Catalog #1: bsm-0978m-hrp-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: HRP

Catalog #1: bsm-0978m-bf350-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: AbBy Fluor-350

Catalog #1: bsm-0978m-apc-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: APC-Cy7

Catalog #1: bsm-0978m-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: Cy7

Catalog #1: bsm-0978m-bf680-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: AbBy Fluor-680

Catalog #1: bsm-0978m-fitc-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: FITC

Catalog #1: bsm-0978m-apc-cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: APC-Cy5.5

Catalog #1: bsm-0978m-rbitc-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: RBITC

Catalog #1: bsm-0978m-pe-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: PE

Catalog #1: bsm-0978m-bf405-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: AbBy Fluor-405

Catalog #1: bsm-0978m-biotin-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: Biotin

Catalog #1: bsm-0978m-percp-cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-0978m-bf555-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: AbBy Fluor-555

Human HepG2 cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Human A549 cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: mouse placenta; Lane 2: 293T cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: A549 cell lysates; Lane 2: 293T cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Hela cell lysates probed with GAPDH(3E12) Monoclonal Antibody, Unconjugated (bsm-0978M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0978m-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: Unconjugated

Catalog #1: bsm-0978m-apc-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: APC

Catalog #1: bsm-0978m-cy3-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: Cy3

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at room temperature\r\nSecondary Ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: MCF-7, BRL, C2C12, COS-7, N2a\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Tissue: Human neuroblastoma\r\nSection type: Formalin-fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:200\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60140M

Catalog #1: bsm-60140m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, C2C12, COS\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Tissue: Human neuroblastoma\r\nSection type: Formalin-fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:200\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60136M

Catalog #1: bsm-60136m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, C2C12, COS\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Tissue: Human neuroblastoma\r\nSection type: Formalin-fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:200\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60138M

Catalog #1: bsm-60138m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: MCF-7, BRL, N2a, COS-7, Mouse liver\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 11 kDa\r\nObserved MW: 11 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Trichostain A (2 \u03bcM, 4 hours)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 11 kDa\r\nObserved MW: 11 kDa
Tissue: Human neuroblastoma\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:500\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60214M\r\n

Catalog #1: bsm-60214m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, C2C12, COS-7, BRL\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 14 kDa\r\nObserved MW: 14 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: Mouse spleen, Mouse liver\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 14 kDa\r\nObserved MW: 14 kDa

Catalog #1: bsm-60139M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60137M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60160M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60148M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60135M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60142M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60143M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60155M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60157M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60134M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: MCF-7, C2C12, COS\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: PTM-669, PTM-669\u00b1H3K56me1 peptide, PTM-669\u00b1H3K56me2 peptide, 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: MCF-7, COS\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa

Catalog #1: bsm-60144M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60158M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60316m-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51630m

Applications: WB

Reactivity: Human, Mouse, Monkey

Conjugate: Unconjugated

Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with ER alpha (5G7) Monoclonal Antibody (bsm-53007M) at a concentration of 7 \u03bcg\/ ml. Figure shows that the antibody is specific for ER alpha isoform, whereas the ER beta isoform is not recognized.
COS-7 cells transiently over-expressing human ER\u03b1 (left) or ER\u00df1 (right) were labeled with the ER alpha (5G7) Monoclonal Antibody (bsm-53007M), used at a concentration of 15 \u03bcg\/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure shows the specificity of the antibody for the ER alpha isoform.
ChIP assays was performed using MCF7 cells treated with the ER agonist estradiol for 3 hours prior to harvesting, ER alpha (5G7) Monoclonal Antibody (bsm-53007M), and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 \u03bcg of antibody were used per ChIP experiment. Recovery (%: ChIP\/input) and occupancy (x fold: +ve\/-ve) are shown. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene used as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +\/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.

Catalog #1: bsm-53007M

Applications: WB, IHC-P, IHC, dot-blot

Reactivity: Human, Monkey

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with YY1 (3C6) Monoclonal Antibody (bsm-51412M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Daudi cell lysates; Lane 2: U-251 cell lysates; probed with YY1 (3C6) Polyclonal Antibody, unconjugated (bsm-51412M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Monkey brain tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with YY1 (3C6) Monoclonal Antibody, Unconjugated (bsm-51412M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51412M

Applications: WB, FCM, IHC-P

Reactivity: Human, Rat, Monkey

Conjugate: Unconjugated