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Western Blot Antibodies



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Catalog #1: bs-16605r-cy3-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-16605r-apc-cy55-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-16605r-apc-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-16605r-apc-cy7-100ul

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-16605r-bf750-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-16605r-bf488-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-16605r-cy5-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-16605r-bf405-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-1651r-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: RBITC

Catalog #1: bs-1651r-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE-Cy7

Catalog #1: bs-1651r-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: FITC

Catalog #1: bs-1651r-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy7

Catalog #1: bs-16605r-bf680-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-16605r-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1651r-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-405

Catalog #1: bs-1651r-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy3

Catalog #1: bs-1651r-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE

Catalog #1: bs-1651r-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-555

Catalog #1: bs-1651r-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE-Cy5

Catalog #1: bs-1651r-biotin-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Biotin

Catalog #1: bs-1651r-pe-cy55-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE-Cy5.5

Catalog #1: bs-1651r-cy55-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5.5

Catalog #1: bs-1651r-hrp-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: HRP

Catalog #1: bs-1651r-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-594

Catalog #1: bs-1651r-cy5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5

Catalog #1: bs-1651r-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-647

Catalog #1: bs-1651r-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-750

Catalog #1: bs-1651r-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-680

Catalog #1: bs-1651r-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-350

Catalog #1: bs-15963r-pe-cy55-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-15592r-hrp-100ul

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-15592r-cy5-100ul

Applications: WB

Reactivity: Mouse

Conjugate: Cy5

Catalog #1: bs-15963r-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bs-1651r-apc-cy55-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: APC-Cy5.5

Catalog #1: bs-15963r-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-15592r-pe-cy5-100ul

Applications: WB

Reactivity: Mouse

Conjugate: PE-Cy5

Catalog #1: bs-15592r-pe-100ul

Applications: WB

Reactivity: Mouse

Conjugate: PE

Lane 1: Mouse Small intestine lysates; Lane 2: Mouse Large intestine lysates probed with AKT1\/3 (Tyr473) Polyclonal Antibody, Unconjugated (bs-15963R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with phospho-AKT3 (Tyr473) . Antibody(bs-15963R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-AKT3 (Tyr473)) polyclonal Antibody, Unconjugated (bs-15963R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-15963r-100ul

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1651r-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: APC-Cy7

Catalog #1: bs-15963r-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-15592r-pe-cy7-100ul

Applications: WB

Reactivity: Mouse

Conjugate: PE-Cy7

Catalog #1: bs-15963r-percp-cy55-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-15963r-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-15963r-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-15592r-rbitc-100ul

Applications: WB

Reactivity: Mouse

Conjugate: RBITC

Catalog #1: bs-15963r-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-15963r-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-15963r-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PE