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Western Blot Antibodies



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HT-1080 Cells lysates probed with MMP14 (3A6) Monoclonal Antibody, unconjugated (bsm-51028M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at Room Temperature.
Lane 1: A2058 Cell lysates; probed with MMP14 (3A6) Monoclonal Antibody, unconjugated (bsm-51028M) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 cells; Antibody incubation with MMP14 (3A6) Monoclonal Antibody, Unconjugated (bsm-51028M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary DAPI and for nuclei staining.

Catalog #1: bsm-51028M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

The TT (treated with BFA ) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-CALCA antibody (bsm-51512M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
25 ug total protein per lane of various lysates (see on figure) probed with CALCA monoclonal antibody, unconjugated (bsm-51512M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-51512m-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52042m-100ul

Applications: WB, ICC/IF

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bs-0369M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: HRP

Catalog #1: bsm-1278m-bf555-100ul

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-555

Catalog #1: bsm-51197m-100ul

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0369M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-51093M

Applications: WB, IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: 293 Cell lysates; Lane 2: K562 Cell lysates; Lane 3: MCF-7 Cell lysates; Lane 4: SK-BR-3 Cell lysates; Lane 5: T47D Cell lysates; Lane 6: MDA-MB-453 Cell lysates; Lane 7: mouse testis lysates; probed with ACE2 (881CT16.4.4) Monoclonal Antibody, unconjugated (bsm-51221M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded (Human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACE2(1C4) ) Monoclonal Antibody, Unconjugated (bsm-51221M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACE2(1C4) ) Monoclonal Antibody, Unconjugated (bsm-51221M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACE2(1C4) ) Monoclonal Antibody, Unconjugated (bsm-51221M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-51221M

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51168M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0369M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rabbit

Conjugate: Biotin

Catalog #1: bsm-1278m-percp-cy5.5-100ul

Applications: IF

Reactivity: Species independent

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-1278m-rbitc-100ul

Applications: IF

Reactivity: Species independent

Conjugate: RBITC

Catalog #1: bsm-1278m-bf750-100ul

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-750

Catalog #1: bsm-1278m-cy7-100ul

Applications: IF

Reactivity: Species independent

Conjugate: Cy7

Catalog #1: bsm-1278m-percp-100ul

Applications: IF

Reactivity: Species independent

Conjugate: PerCP

Catalog #1: bsm-1278m-cy5-100ul

Applications: IF

Reactivity: Species independent

Conjugate: Cy5

Catalog #1: bsm-1278m-pe-100ul

Applications: IF

Reactivity: Species independent

Conjugate: PE

Catalog #1: bsm-1278m-hrp-100ul

Applications: ELISA, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-1278m-apc-cy5.5-100ul

Applications: IF

Reactivity: Species independent

Conjugate: APC-Cy5.5

Catalog #1: bsm-1278m-biotin-100ul

Applications: ELISA, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-1278m-bf594-100ul

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-594

Catalog #1: bsm-1278m-cy3-100ul

Applications: IF

Reactivity: Species independent

Conjugate: Cy3

Catalog #1: bsm-1278m-cy5.5-100ul

Applications: IF

Reactivity: Species independent

Conjugate: Cy5.5

Catalog #1: bsm-1278m-fitc-100ul

Applications: IF

Reactivity: Species independent

Conjugate: FITC

Catalog #1: bsm-1278m-bf680-100ul

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-680

Catalog #1: bsm-1278m-bf647-100ul

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-647

Catalog #1: bsm-1278m-bf405-100ul

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-405

Catalog #1: bsm-1278m-apc-100ul

Applications: IF

Reactivity: Species independent

Conjugate: APC

Catalog #1: bsm-1278m-bf488-100ul

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-488

Catalog #1: bsm-1278m-bf350-100ul

Applications: IF

Reactivity: Species independent

Conjugate: AbBy Fluor-350

200 ng 8-OHdG-BSA protein (bs-1278PB) per lane probed with DNA\/RNA Damage monoclonal antibody respectively, unconjugated (bsm-1278M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 8-OHdG (DNA\/RNA Damage) Monoclonal Antibody, Unconjugated (ascites of bsm-1278M) at 1:5000 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 8-OHdG (DNA\/RNA Damage) Monoclonal Antibody, Unconjugated (ascites of bsm-1278M) at 1:5000 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-1278m-100ul

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-34215m-100ul

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-70271M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine, Pig, Rabbit, Hamster

Conjugate: Unconjugated

Catalog #1: bsm-70417M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-70034M

Applications: WB, IHC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70126M

Applications: WB, IHC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bsm-70311M

Applications: WB, IHC

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human breast ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (FASN (497CT15.2.5)) Monoclonal Antibody, Unconjugated (bsm-51155M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (FASN (497CT15.2.5)) Monoclonal Antibody, Unconjugated (bsm-51155M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (FASN (497CT15.2.5)) Monoclonal Antibody, Unconjugated (bsm-51155M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (FASN (497CT15.2.5)) Monoclonal Antibody, Unconjugated (bsm-51155M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-51155m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0370M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: HRP

Catalog #1: bs-0370M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Goat

Conjugate: Biotin

Catalog #1: bs-0370M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Goat

Conjugate: Unconjugated

Catalog #1: bs-0346M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-0346M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-0346M

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Lane 1: HepG2 cell lysates probed with THAP11 Monoclonal Antibody, Unconjugated (bsm-51505M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: NIH\/3T3 cell lysates probed with THAP11 Monoclonal Antibody, Unconjugated (bsm-51505M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51505m

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP3A4 Monoclonal Antibody, Unconjugated (bsm-51529M) at 1:400 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51529m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51522m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated