IKB epsilon Polyclonal Antibody

$Lane 1: mouse brain lysates Lane 2: mouse heart lysates probed with Anti NFKBIE\/IKB epsilon Polyclonal Antibody, Unconjugated (bs-4139R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 55kD. Observed band size: 55kD.$

Lane 1: mouse brain lysates Lane 2: mouse heart lysates probed with Anti NFKBIE\/IKB epsilon Polyclonal Antibody, Unconjugated (bs-4139R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 55kD. Observed band size: 55kD.

$Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-IKB epsilon Polyclonal Antibody, Unconjugated (bs-4139R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-IKB epsilon Polyclonal Antibody, Unconjugated (bs-4139R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Lane 1: A431 cell lysates probed with IKB epsilon Polyclonal Antibody, Unconjugated (bs-4139R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: A431 cell lysates probed with IKB epsilon Polyclonal Antibody, Unconjugated (bs-4139R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Lane 1: Spleen lysates probed with IKB epsilon Polyclonal Antibody, Unconjugated (bs-4139R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Spleen lysates probed with IKB epsilon Polyclonal Antibody, Unconjugated (bs-4139R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bs-4139R
Product Name	IKB epsilon Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human IKB epsilon
Immunogen Range	165-270/500
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4794
Swiss Prot	O00221
Subcellular location	Cytoplasm
Synonyms	IKBE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; I-kappa-B-epsilon; IkB-E; IkB-epsilon; IkappaBepsilon; NFKBIE
Background	Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200