EHMT1 Polyclonal Antibody

To determine the titer, an ELISA was performed using a serial dilution of the EHMT1 antibody (bs-53068R). The wells were coated with peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,000.

$Nuclear extracts of HeLa cells (40 \u03bcg) were analyzed by Western blot using the EHMT1 antibody (bs-53068R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment may represent a splicing variant.$

Nuclear extracts of HeLa cells (40 \u03bcg) were analyzed by Western blot using the EHMT1 antibody (bs-53068R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment may represent a splicing variant.

Applications

WB
ELISA
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bs-53068R
Product Name	EHMT1 Polyclonal Antibody
Applications	WB ELISA IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Polyclonal antibody raised in rabbit against mouse Ehmt1, using three KLH-conjugated synthetic peptides containing a sequence from the central part of the protein
Clonality	Polyclonal
Isotype	Not Determined
Concentration	Not Determined
Purification	Whole antiserum from rabbit containing 0.05% azide
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	79813
Swiss Prot	Q9H9B1
Synonyms	Histone-lysine N-methyltransferase EHMT1, EHMT1, GLP1, Lysine N-methyltransferase 1D, H3-K9-HMTase 5,
Background	Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400