CBX3 Polyclonal Antibody

To determine the titer of the antibody, an ELISA was performed using a serial dilution of the CBX3 antibody (bs-53080R). The plates were coated with peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3,250

Applications

WB
ELISA

Reactivity

Human

Overview
Catalog #	bs-53080R
Product Name	CBX3 Polyclonal Antibody
Applications	WB ELISA
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Polyclonal antibody raised in rabbit against human CBX3 using two KLH-conjugated synthetic peptides containing a sequence from the N-terminal and the central region of the protein, respectively
Clonality	Polyclonal
Isotype	Not Determined
Concentration	Not Determined
Purification	Whole antiserum from rabbit containing 0.05% azide
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	11335
Swiss Prot	Q13185
Synonyms	Chromobox protein homolog 3, CBX3, HECH, Heterochromatin protein 1 homolog gamma, HP1 gamma, Modifier 2 protein
Background	Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000