_1-Catenin (N-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70377r |
| Product Name | _1-Catenin (N-terminal region) Antibody |
| Applications |
WB
|
| Specificity | The antibody detects a 102 kDa* protein corresponding to the molecular mass of _1-Catenin on SDS-PAGE immunoblots of rat PC12 and mouse SYF cells. This peptide sequence is highly conserved in rat and mouse _1-Catenin, and has some homology to _2-Catenin or _3-Catenin. |
| Reactivity | Human, Mouse, Rat, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | _1-Catenin synthetic peptide (coupled to KLH) corresponding to amino acid residues from the N-terminal region of human _1-Catenin. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target | |
| Swiss Prot | P35221 |
| Synonyms | alphaE-catenin, catenin alpha1, catenin |
| Background | _-catenins are cadherin interacting proteins with homology to vinculin. Three _-catenin genes have been described including _1-catenin (_E-Catenin), _2-catenin (_N-catenin), and _3-catenin (_T-catenin). _1-catenin has 81% homology with _2-catenin and 60% homology with _3-catenin. These _-catenin isoforms may have similar roles since each binds cadherins. However, their expression patterns are both overlapping and distinct. _1-catenin was identified in epithelial cells, and is expressed in various cell types. _2-catenin is enriched in the nervous system, and _3-catenin is expressed highest in testis and heart. Phosphorylation may regulate the activity of _1-catenin, since tyrosine phosphorylation of Tyr-148 occurs during intercellular adhesion. This site is dephosphorylated by SHP2, which inhibits _1-catenin binding to _-catenin and translocation to the plasma membrane. Phosphorylation of _1-catenin at Tyr-148 may be important for inhibition of cell transformation, and dephosphorylation of this site may be important during SHP2-mediated cell transformation. |
| Application Dilution | |
| WB | 1:300-5000 |