_1-Catenin (Tyr-148), Phosphospecific Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70378r |
| Product Name | _1-Catenin (Tyr-148), Phosphospecific Antibody |
| Applications |
WB
|
| Specificity | This antibody was cross-adsorbed to unrelated phospho-tyrosine peptide before affinity purification using phospho-α1-Catenin (Tyr-148) peptide (without carrier). The antibody detects a 102 kDa* protein corresponding to the molecular mass of α1-Catenin on SDS-PAGE immunoblots of rat PC12 cells treated with pervanadate. This peptide sequence is highly conserved in rat and mouse α1-Catenin, but is not conserved in α2-Catenin or α3-Catenin. |
| Reactivity | Human, Mouse, Rat, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Phospho-α1-Catenin (Tyr-148) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 148 in human α1-Catenin. |
| Modification Site | Tyr-148 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Antigen Affinity purification |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P35221 |
| Synonyms | alphaE-catenin, catenin alpha1, catenin |
| Background | α-catenins are cadherin interacting proteins with homology to vinculin. Three α-catenin genes have been described including α1-catenin (αE-Catenin), α2-catenin (αN-catenin), and α3-catenin (αT-catenin). α1-catenin has 81% homology with α2-catenin and 60% homology with α3-catenin. These α-catenin isoforms may have similar roles since each binds cadherins. However, their expression patterns are both overlapping and distinct. α1-catenin was identified in epithelial cells, and is expressed in various cell types. α2-catenin is enriched in the nervous system, and α3-catenin is expressed highest in testis and heart. Phosphorylation may regulate the activity of α1-catenin, since tyrosine phosphorylation of Tyr-148 occurs during intercellular adhesion. This site is dephosphorylated by SHP2, which inhibits α1-catenin binding to β-catenin and translocation to the plasma membrane. Phosphorylation of α1-catenin at Tyr-148 may be important for inhibition of cell transformation, and dephosphorylation of this site may be important during SHP2-mediated cell transformation. |
| Application Dilution | |
| WB | 1:300-5000 |