PRKAA1 (7C6) Monoclonal Antibody

$Lane 1: MCF-7; Lane 2: MDA-MB-468 whole cell lysate probed with bsm-51040M PRKAA1 (167CT22.1.6) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: MCF-7; Lane 2: MDA-MB-468 whole cell lysate probed with bsm-51040M PRKAA1 (167CT22.1.6) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Applications

WB
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-51040M
Product Name	PRKAA1 (7C6) Monoclonal Antibody
Applications	WB IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This PRKAA1 Monoclonal antibody was raised using purified His-tagged recombinant human PRKAA1.
Clonality	Monoclonal
Clone #	7C6
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	5562
Swiss Prot	Q13131
Synonyms	AMPK; AMPKa1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA1; AMPK1
Background	Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400