THEM2 (9C3) Monoclonal Antibody

$Lane 1: HepG2; Lane 2: Hela whole cell lysate at 20\u00b5g per lane, probed with bsm-51041M THEM2 (169CT7.1.3) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: HepG2; Lane 2: Hela whole cell lysate at 20\u00b5g per lane, probed with bsm-51041M THEM2 (169CT7.1.3) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51041M
Product Name	THEM2 (9C3) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This THEM2 monoclonal antibody is generated from mouse immunized with THEM2 recombinant protein.
Clonality	Monoclonal
Clone #	9C3
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	55856
Swiss Prot	Q9NPJ3
Synonyms	HT012; THEM2; PNAS-27; Acyl-coenzyme A thioesterase 13; Acyl-CoA thioesterase 13; Thioesterase superfamily member 2; ACOT13
Background	Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro). May play a role in controlling adaptive thermogenesis (By similarity).
Application Dilution
WB	1:300-5000