SUMO2 (7A3) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51241M |
| Product Name | SUMO2 (7A3) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IF
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Purified His-tagged SUMO2 protein was used to produced this monoclonal antibody. |
| Clonality | Monoclonal |
| Clone # | 7A3 |
| Isotype | IgG2b |
| Concentration | 0.5ug/ul |
| Purification | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 6613 |
| Swiss Prot | P61956 |
| Synonyms | HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2; Small ubiquitin-related modifier 2; SUMO-2; SMT3 homolog 2; SUMO-3; Sentrin-2; Ubiquitin-like protein SMT3B; SUMO2 |
| Background | Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed:26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed:18408734, PubMed:18538659, PubMed:21965678, PubMed:9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IF | |