VRK1 (5C1) Monoclonal Antibody

$Lane 1: HeLa Cell lysates; Lane 2: HL-60Cell lysates; probed with VRK1 (1015CT2.1.1) Monoclonal Antibody, unconjugated (bsm-51242M) at 1:5000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HeLa Cell lysates; Lane 2: HL-60Cell lysates; probed with VRK1 (1015CT2.1.1) Monoclonal Antibody, unconjugated (bsm-51242M) at 1:5000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51242M
Product Name	VRK1 (5C1) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Purified His-tagged VRK1 protein was used to produced this monoclonal antibody.
Clonality	Monoclonal
Clone #	5C1
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	7443
Swiss Prot	Q99986
Synonyms	PCH1; PCH1A; Serine/threonine-protein kinase VRK1; Vaccinia-related kinase 1; VRK1
Background	Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.
Application Dilution
WB	1:300-5000