POU2AF1 (7C1) Monoclonal Antibody

$Lane 1: Daudi Cell lysates; probed with POU2AF1 (1170CT1.1.1) Monoclonal Antibody, unconjugated (bsm-51252M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Daudi Cell lysates; probed with POU2AF1 (1170CT1.1.1) Monoclonal Antibody, unconjugated (bsm-51252M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51252M
Product Name	POU2AF1 (7C1) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Purified His-tagged POU2AF1 protein was used to produced this monoclonal antibody.
Clonality	Monoclonal
Clone #	7C1
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	5450
Swiss Prot	Q16633
Synonyms	BOB1; OBF1; OCAB; OBF-1; POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1; POU2AF1
Background	Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Application Dilution
WB	1:300-5000