USF1 (4C4) Monoclonal Antibody

$Lane 1: HeLa Cell lysates; Lane 2: Daudi Cell lysates; probed with USF1 (4C4) Monoclonal Antibody, unconjugated (bsm-51254M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HeLa Cell lysates; Lane 2: Daudi Cell lysates; probed with USF1 (4C4) Monoclonal Antibody, unconjugated (bsm-51254M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51254M
Product Name	USF1 (4C4) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This USF1 antibody is generated from a mice immunized with a recombinant protein between 1-310 amino acids from the Central region of human USF1.
Clonality	Monoclonal
Clone #	4C4
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	7391
Swiss Prot	P22415
Synonyms	UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11; Upstream stimulatory factor 1; Class B basic helix-loop-helix protein 11; Major late transcription factor 1; USF1; USF
Background	Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Application Dilution
WB	1:300-5000