SRC (17AT28) Monoclonal Antibody

$Lane 1: HT29 Cell lysates; Lane 2: Jurkat Cell lysates; probed with SRC (17AT28) Monoclonal Antibody, unconjugated (bsm-51271M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HT29 Cell lysates; Lane 2: Jurkat Cell lysates; probed with SRC (17AT28) Monoclonal Antibody, unconjugated (bsm-51271M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-51271M
Product Name	SRC (17AT28) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This Src antibody is generated from mouse immunized with a recombinant protein of full length human SRC.
Clonality	Monoclonal
Clone #	17AT28
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	6714
Swiss Prot	P12931
Synonyms	ASV; SRC1; THC6; c-SRC; p60-Src; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; SRC
Background	This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Application Dilution
WB	1:300-5000