MCM3 (3C3) Monoclonal Antibody

$Lane 1: CHO; Lane 2: NIH\/3T3; Lane 3: Hela; Lane 4: HepG2; Lane 5: C2C12; Lane 6: PC-12 cell lysates; probed with MCM3 (3C3) Polyclonal Antibody, unconjugated (bsm-51337M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: CHO; Lane 2: NIH\/3T3; Lane 3: Hela; Lane 4: HepG2; Lane 5: C2C12; Lane 6: PC-12 cell lysates; probed with MCM3 (3C3) Polyclonal Antibody, unconjugated (bsm-51337M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51337M
Product Name	MCM3 (3C3) Monoclonal Antibody
Applications	WB FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This MCM3 antibody is generated from a mouse immunized with a recombinant protein of human MCM3.
Clonality	Monoclonal
Clone #	3C3
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	4172
Swiss Prot	P25205
Synonyms	HCC5; P1.h; RLFB; P1-MCM3; DNA replication licensing factor MCM3; DNA polymerase alpha holoenzyme-associated protein P1; RLF subunit beta; p102; MCM3
Background	Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Application Dilution
WB	1:300-5000
FCM	1:20-100