PDIA1 (3C3) Monoclonal Antibody

$Lane 1: HeLa; Lane 2: MCF-7; Lane 3: HepG2; Lane 4: PANC-1 cell testis lysate (20\u00b5g) probed with bsm-51338M PDIA1 (3C3) Monoclonal Antibody at 1:4000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: HeLa; Lane 2: MCF-7; Lane 3: HepG2; Lane 4: PANC-1 cell testis lysate (20\u00b5g) probed with bsm-51338M PDIA1 (3C3) Monoclonal Antibody at 1:4000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51338M
Product Name	PDIA1 (3C3) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This PDIA1 antibody is generated from a mouse immunized with a recombinant protein of human PDIA1.
Clonality	Monoclonal
Clone #	3C3
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	5034
Swiss Prot	P07237
Synonyms	DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; CLCRP1; ERBA2L; P4Hbeta; Protein disulfide-isomerase; Cellular thyroid hormone-binding protein; Prolyl 4-hydroxylase subunit beta; p55; P4HB
Background	This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).
Application Dilution
WB	1:300-5000