BANP (4C2) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with BANP (4C2) Monoclonal Antibody (bsm-51349M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with BANP (4C2) Monoclonal Antibody (bsm-51349M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Applications

WB
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-51349M
Product Name	BANP (4C2) Monoclonal Antibody
Applications	WB IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This BANP antibody is generated from a mouse immunized with a recombinant protein between 30-390 amino acids from human BANP.
Clonality	Monoclonal
Clone #	4C2
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	54971
Swiss Prot	Q8N9N5
Synonyms	BEND1; SMAR1; SMARBP1; Protein BANP; BEN domain-containing protein 1; Btg3-associated nuclear protein; Scaffold/matrix-associated region-1-binding protein; BANP
Background	Controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function. Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 'Ser-15' phosphorylation and nuclear accumulation, which causes cell cycle arrest (By similarity).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400